FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005765950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005765950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518633
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG600.0132.08
TCGGCGG1250.063.3599979
TATCGGC1450.054.6206937
TTATCGG1550.051.0967756
ATTATCG2601.200533E-1030.4615385
GCGATCG550.00276177215.709091130-134
CCGGCGA1251.1541488E-814.97630-34
CGGCGAG1354.7684625E-712.830-34
GTTACCG1354.7684625E-712.820-24
TCCGGCG1409.964126E-611.31428625-29
AATCCGG1655.34273E-610.47272725-29
TTACCGA1953.714773E-69.620-24
ATCCGGC1656.1030514E-59.59999925-29
CGTTACC2005.1272054E-69.36000115-19
GGTTCGT1852.079914E-59.3405415-19
TCGTTAC2056.950176E-58.42926915-19
GGGTTCG2109.207661E-58.22857110-14
TACCGAA2302.990747E-58.1391320-24
GCGGGTT2004.913589E-47.9210-14
GAATCCG2252.0511376E-47.6825-29
Produced by FastQC (version 0.11.5)