FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005766009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005766009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261100
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG451.2848777E-680.08
TATCGGC1102.5375225E-432.7272727
TCGGCGG1358.41732E-426.6666669
TTATCGG1500.001555940724.06
ATTATCG1900.006128863418.9473695
CTCGTCG853.5506673E-818.63529415-19
TCGTCGC906.828486E-817.615-19
CGTCGCT1252.8139948E-612.67215-19
CAGTACC7551.3714816E-712.3973528
CCAGTAC7952.6833186E-711.7735847
AGTACCT8851.0724034E-610.5762719
TCCAGTA11502.9182047E-810.017396
AGACCTC10206.550571E-58.4705881
GACCTCC9653.3443185E-48.2072532
ACCGTAA2201.581142E-47.854545635-39
CACCGTA2456.576134E-57.640816735-39
ACCTCCA15352.3944522E-67.5048863
CTCCAGT16658.052379E-66.9189195
CCTCGTC3202.5042173E-46.315-19
CCTCCAG18604.106477E-56.19354874
Produced by FastQC (version 0.11.5)