FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005766109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005766109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017524
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGG1400.082.285719
ATCGGCG1400.082.285718
TATCGGC2400.048.07
TTATCGG2700.039.9999966
ATTATCG4151.8189894E-1224.2891565
TCGTCGC1800.016.015-19
GCGATCG757.976183E-515.359999130-134
CAGTACC11650.014.8326188
CTCGTCG2100.013.71428715-19
CCGATCG650.00837191413.29230955-59
CGTCGCT1951.2732926E-1113.29230815-19
CCGGCGA1806.2937033E-1012.830-34
CCAGTAC12300.012.2926837
GTTACCG2053.8380676E-1011.941462520-24
AGTACCT13900.011.3956849
CGGCGAG2158.4764906E-1011.38604630-34
AGACCTC16300.010.6012271
AATCCGG2201.4688339E-810.47272725-29
CGATCGG1100.002015391410.472727130-134
GACCTCC16000.010.3499992