FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005766355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005766355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3188373
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1700.093.176478
TCGGCGG2850.055.5789459
TATCGGC3250.053.169237
TTATCGG3550.034.4788746
ATTATCG5450.022.4587155
TCGTCGC1751.8189894E-1214.81142815-19
CTCGTCG1855.456968E-1214.01081115-19
TCCGGCG2650.011.95471725-29
CGTCGCT2652.0008883E-1110.86792515-19
CGATCGG1100.002015502210.472727130-134
GTTACCG2901.0913936E-1110.42758720-24
CCGATCG1254.994945E-410.36855-59
CGGCGAG3251.8189894E-1210.19076930-34
CCGGCGA2951.6298145E-99.76271255
CGTTACC3155.4569682E-119.615-19
TCGTTAC3504.5474735E-119.05142915-19
CCAGTAC18451.4551915E-118.975617
CAGTACC17503.97813E-98.2285718
TTACCGA4007.1304385E-107.9220-24
TACCGAA4007.1304385E-107.9220-24
Produced by FastQC (version 0.11.5)