FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005766408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005766408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7790998
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2100.054.8571438
TCGGCGG4701.0913936E-1121.4468089
TATCGGC5505.456968E-1219.6363667
TTATCGG7151.6370905E-1116.1118876
CCGATCG1708.6710765E-48.47058855-59
CGATCGC2457.404551E-68.22857160-64
TCGTCGC2857.83788E-78.0842115-19
ATTATCG16451.0159965E-77.8784195
CCGGCGA3801.8404535E-87.578947530-34
CGATCGG2000.00428811377.2000003130-134
CTCGTCG3501.6872269E-56.58285715-19
TCCGGCG4902.851648E-76.17142825-29
CGGCGAG5453.3640754E-75.81284430-34
CGTCGCT4404.599397E-45.236363415-19
ATCCGGC6655.1730603E-75.196992425-29
GTTACCG6757.031176E-75.1220-24