FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005766591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005766591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7360560
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG3350.077.373148
TCGGCGG5900.043.9322059
TATCGGC7600.033.1578947
TTATCGG7850.025.6815286
ATTATCG11600.018.620695
TCGTCGC3150.012.34285715-19
CTCGTCG4100.010.18536715-19
CCGGCGA5250.09.87428630-34
CGTCGCT4550.09.17802215-19
CGGCGAG6050.09.04462830-34
TCCGGCG5600.09.025-29
CCGATCG2303.1626714E-68.76521755-59
ATCCGGC7300.07.101370325-29
CGATCGG3101.659913E-46.5032263130-134
AATCCGG8150.06.18404925-29
CCAGTAC39950.06.12765937
GTTACCG6451.2332748E-96.027907420-24
CGTTACC7301.05501385E-105.917808515-19
CAGTACC41257.6397555E-115.58545458
CGATCGC3150.0091675695.485714460-64