FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005766643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005766643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6083262
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG4200.077.142858
TCGGCGG6550.048.366419
TATCGGC7600.041.6842087
TTATCGG8900.033.977536
ATTATCG12950.023.351355
TCGTCGC4650.013.62580715-19
CCGATCG2351.8189894E-1212.2553255-59
CTCGTCG5200.012.18461515-19
TCCGGCG6300.011.65714325-29
CCGGCGA6050.011.18677730-34
CGTCGCT5400.010.93333215-19
CGGCGAG6700.010.74626930-34
CGATCGG2457.3123374E-99.9918375130-134
GTTACCG6200.09.52258120-24
CCAGTAC37100.09.3153647
CGATCGC3104.0563464E-109.29032360-64
ATCCGGC7950.09.23773725-29
CGTTACC7450.08.89127515-19
TCGTTAC7350.08.81632615-19
TTACCGA7200.08.620-24