FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005766722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005766722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661662
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1150.0118.956528
TCGGCGG1800.076.09
TATCGGC2200.065.454547
TTATCGG2150.060.279076
ATTATCG3750.034.565
CCGATCG751.9490653E-719.255-59
CGATCGG751.9490653E-719.2130-134
CTCGTCG1055.4569682E-1119.19999915-19
TCGTCGC1005.966285E-1018.7215-19
GCGATCG857.248036E-716.941175130-134
GTTACCG1800.016.820-24
CGATCGC807.637635E-616.260-64
TCCGGCG1800.015.225-29
CCGGCGA1800.015.230-34
ATCCGGC1800.015.225-29
CGTCGCT1301.9352228E-814.40000115-19
CGTTACC1950.014.03076915-19
CGGCGAG2155.456968E-1212.72558130-34
GGTTCGT2750.012.56727315-19
AATCCGG2251.0913936E-1112.1625-29