FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005766776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005766776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3222569
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCTCCAGTACCTCCTCGTCGCTGGTATAACCTTCACCGTAAAAGCTC32780.10172008729681195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG5050.0121.188128
TATCGGC5450.0112.293577
TCGGCGG6000.0101.999999
TTATCGG6850.086.189786
ATTATCG8850.066.711865
CCAGTAC22400.037.6071437
CAGTACC23300.036.1545078
GACCTCC26150.032.214152
AGTACCT27800.030.0431659
TCCAGTA30450.028.6108386
AGACCTC31600.027.1139241
TCGTCGC6600.025.0909115-19
CTCGTCG6650.024.90225615-19
CCGATCG3650.024.46027455-59
CGATCGG2700.022.933332130-134
CGTCGCT7500.022.08000215-19
CGATCGC3950.021.87341760-64
TCCGGCG5550.020.75675825-29
GCGATCG2850.020.715788130-134
GCTATTA29050.020.323582
Produced by FastQC (version 0.11.5)