FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005766967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005766967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230541
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1600.067.500018
TATCGGC2350.045.9574477
TCGGCGG2250.044.89
TTATCGG3200.031.5000026
ATTATCG4952.3646862E-1120.3636365
CGATCGG852.3121736E-413.552941130-134
GCGATCG1201.7803832E-613.200001130-134
CTCGTCG2103.6379788E-1213.02857115-19
TCGTCGC2001.8189894E-1112.9615-19
CCGATCG903.7492072E-412.855-59
CAGTACC12951.8189894E-1211.6756768
CCGGCGA2054.8767106E-911.23902430-34
CGTCGCT2850.011.11578915-19
CCAGTAC13653.6379788E-1211.0769237
CGTTACC2151.1751763E-710.04651215-19
TCCGGCG2201.643366E-79.81818225-29
AGTACCT18600.09.677429
GCTATTA17002.5465852E-119.3176472
GACCTCC16801.9463187E-109.02
ATCCGGC2754.8046786E-88.90181925-29
Produced by FastQC (version 0.11.5)