FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005767015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005767015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343800
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1400.061.7142838
TCGGCGG2551.8189894E-1233.8823559
TATCGGC2603.6379788E-1233.230777
TTATCGG2901.2732926E-1129.7931026
ATTATCG4352.1445885E-919.8620685
TCGTCGC1856.91216E-1113.23243215-19
CGTCGCT2155.456968E-1212.72558115-19
CGAAGGT4000.002252726312.5999992
CTCGTCG2404.5474735E-1010.815-19
CAGTACC13452.7284841E-1110.706328
CCAGTAC13955.4569682E-1110.322587
CCGGCGA1851.8963037E-610.11891930-34
TCCGGCG2354.2267147E-68.57872425-29
GTTACCG2353.914184E-57.965957620-24
AGACCTC18257.248491E-87.49589061
GACCTCC17452.72943E-77.4269342
AGTACCT16951.3636472E-67.22123869
TCGTTAC3100.0076858026.96774246
CGTTACC2506.878539E-46.911999715-19
ATCCGGC3152.7675533E-56.85714325-29
Produced by FastQC (version 0.11.5)