FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005767179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005767179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7202170
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG3300.063.2727248
TATCGGC7350.029.3877547
TCGGCGG7150.029.2027999
TTATCGG8700.023.1724136
ATTATCG10900.017.8348645
TCGTCGC4200.010.97142815-19
CCGATCG3052.9831426E-109.44262355-59
CGATCGG2858.5630745E-88.589474130-134
CTCGTCG5750.07.51304415-19
CGTCGCT6000.07.4415-19
CCGGCGA5751.8189894E-127.26260930-34
GCGATCG3952.8971226E-76.9265823130-134
CGATCGC4003.605437E-76.8460-64
GTTACCG6003.092282E-116.720000320-24
CCAGTAC42450.06.10600767
TTACCGA7402.3646862E-116.032432620-24
TCCGGCG7503.45608E-115.951999725-29
CAGTACC41201.8189894E-125.9417488
CGGCGAG6753.603418E-95.7630-34
CCGAATC9603.092282E-115.2520-24
Produced by FastQC (version 0.11.5)