FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6341584
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC720491.1361357036349278No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT518700.8179344466619066No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC515020.8121314800844709No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC464490.7324510721611509No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT359310.5665934567767296No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC330840.5216993104561888No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA319470.5037700360036231No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC298760.4711125800746312No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC268610.4235692533600438No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT263410.41536940928323274No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT235000.3705698765481936No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC219140.3455603521139198No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG204220.3220331071858388No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC200760.316577057088576No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG198750.31340750197427014No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT190700.3007135125861299No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA179020.28229540127513886No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG177150.2793466111936702No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT174600.2753255338098494No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA168240.26529649374667275No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT167790.26458689185541023No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG154450.2435511380122064No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC153980.24280999825911004No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA149820.23625012299766115No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG139420.219850434844039No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC137250.21642857683506203No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA133600.21067291705037733No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA130140.20521686695311453No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA129630.20441265147635038No Hit
ACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA127420.20092771774370566No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA126740.19985543044135343No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA125730.19826276841874205No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG124920.19698548501446955No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT124520.19635472777779178No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA122570.19327978624898765No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA121020.19083560195686125No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA115060.18143731913036237No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC114480.1805227211371796No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG112450.17732162816103988No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG98440.15522935594640078No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC97920.15440937153871967No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG97290.15341592889095218No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG96150.1516182707664205No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG95940.15128712321716467No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC92630.14606760708365607No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT92430.14575222846531719No Hit
TCGATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA91890.14490070619580217No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA91330.1440176460644533Illumina RNA PCR Primer (96% over 28bp)
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG88380.13936581144395468No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC88200.1390819706874497No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC85360.13460359430703747No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA84910.133893992415775No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG84550.13332631090276498No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC84350.1330109322844261No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC80920.12760218897991418No Hit
TGAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCC79270.12500031537861833No Hit
TTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTAT79260.1249845464477014Illumina RNA PCR Primer (96% over 30bp)
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC78980.12454301638202694No Hit
ATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGA78800.12425917562552197Illumina RNA PCR Primer (96% over 26bp)
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG78220.12334457763233918No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC78010.12301343008308335No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG75550.11913427307751502No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC74670.1177466071568239No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC73240.11549165003570086No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT66930.10554145462710893No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT66230.10443762946292282No Hit
TTTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTG65960.10401186832816534Illumina RNA PCR Primer (96% over 30bp)
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA65860.10385417901899588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)