FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880930
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA2434528.450465648245533No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT1495385.190615530401641No Hit
CCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAG772982.683091918234737No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT741932.5753142214493234No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA332101.152752756922244No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG317591.1023870763954695No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA305311.0597619518697088No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC220130.7640935392390652No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT211940.7356652192174056No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA193010.6699572707424338No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG114920.3988989666531294No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA99090.3439514323499703Illumina RNA PCR Primer (96% over 28bp)
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG83330.2892468751410135No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC73910.25654910046408624No Hit
CACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA73380.25470941675084086No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC69250.2403737681929099No Hit
TTTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTG69240.24033905717945245Illumina RNA PCR Primer (96% over 30bp)
CGATCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGA67240.2333968544879605No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC61040.21187602614433534No Hit
ATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGA58160.20187925426858686Illumina RNA PCR Primer (96% over 26bp)
TCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCT56820.19722797846528725No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC56640.19660318022305298No Hit
AACCACAGAGGATTTCCCGCTCAGGCTGCTGGCGGCTGCTCCCTCCCAGA56070.19462465245597776No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA53470.1855997889570382No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA53430.18546094490320833No Hit
TTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTAT50930.17678319153884337Illumina RNA PCR Primer (96% over 30bp)
CGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTA50270.174492264650651Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AACCACAGAGGAGTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA49010.17011867695501107No Hit
GTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTG48820.16945916769931932No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT48500.1683484152686806No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC44770.15540120724904805No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG44590.15477640900681378No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG43430.15074993144574841No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA40410.14026720538159554No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC39020.1354423745110086No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT38860.13488699829568926No Hit
CTCAGGGCGCCAGTGCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT38050.132075406205635No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA36990.12839603877914424No Hit
TTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGT35760.12412658412387667Illumina RNA PCR Primer (96% over 30bp)
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG35400.1228769876394081No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG35000.1214885471011097No Hit
ACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGT34940.12128028102036494Illumina RNA PCR Primer (95% over 24bp)
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC33980.1179480237284488No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG33700.11697611535163993No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA33520.11635131710940563No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA33320.11565709684025645No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA33250.11541411974605423No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC32730.1136091470462663No Hit
ACCCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA32330.11222070650796791No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC32320.11218599549451046No Hit
TGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG32110.1114570642119038No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCGCTGAGATGAATGTGAGCACCT31860.11058928887546729No Hit
GGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTAC30320.10524379280301846Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG29490.1023627786860493No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA28850.10014127382477185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)