FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096897
Sequences flagged as poor quality0
Sequence length115-122
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC2118236.83984646567193No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT1129193.6461981137893833No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC977913.157709152096437No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG774052.499437340021318No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG717452.316673754406427No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC666772.1530260773929517No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT666402.1518313331053633No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT583841.8852419050423699No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT448701.4488696265972036No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG448091.44689991304199No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA427831.3814795906999813No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG285140.9207280707107792No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC284450.9185000340663574No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG272300.8792672148928427No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT272080.8785568263975199No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA243590.7865615162532045No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT236740.7644426017397414No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC211650.6834260228867799No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC197250.636927866829281No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG189950.6133558849390213No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC178730.5771260716775534No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC170650.5510354396675123No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT166610.5379901236624919No Hit
GGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCATGGGTACCAGTC162700.5253645826774349No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA158180.5107693281371644No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT150380.4855828269393525No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTA145460.46969595695304045No Hit
GGACACTGTCATGGGTACCAGTCTCCTATGCTGGGTGGTCCTGGGTTTCC141100.4556173485911866No Hit
AGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT140590.45397053889748346No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT139560.4506446291239263No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTG137070.4426043229723171No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT129400.41783759679446875No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG126020.406923446275417No Hit
GCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT125380.4048568615617504No Hit
GATCCTGGTCTGACACTGTCATGGGTACCAGTCTCCTATGCTGGGTGGTC110820.35784205932583485No Hit
GGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGT101310.327133902096195No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG93590.30220572398759143No Hit
GAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCATGG93180.3008818181553988No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG92120.29745903722338846No Hit
GAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCC90970.2937456428160188No Hit
TGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCC88950.2872229848135085No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC86350.2788274844142379No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG84740.2736287322439203No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC84540.27298292452089945No Hit
GTCTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTG84280.2721433744809724No Hit
GATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGCC83130.26842998007360275No Hit
GGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATG80130.25874286422829046No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC79820.2577418622576082No Hit
GGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCATGGGTA76960.2485068118184105No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA76440.24682771173855636No Hit
GAGACAAATCAGGGCTGCCCAGTGATCGGTTCTCTGCAGAGAGGCCTGAG76310.24640793671859285No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT75800.24476112702488975No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT75040.24230705767741065No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC71180.22984296862310888No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT71110.22961693592005158No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC70180.22661393000800478No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC70000.22603270305728607No Hit
GAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCT67290.217282008410354No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG66710.21540916601359364No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC66680.2153122948551405No Hit
GTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT66220.21382693709219258No Hit
GCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC66120.21350403323068218No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC61520.19865045560120337No Hit
GGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT61070.19719738822440658No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT60320.1947756092630785No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG60200.194388124629266No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGGGCACCAGTC60060.19393605922315144No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATG58560.1890925013004953No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG58310.18828524164671928No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT57040.18418436260553708No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA55230.1783398027121987No Hit
GGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG55190.17821064116759455No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT54270.17523992564169877No Hit
GCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCG53590.17304417938342798No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG53510.17278585629421966No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT53230.17188172548199052No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA53120.17152653123432907No Hit
GTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC52400.16920162343145412No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG52110.16826520223307395No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC51640.166747554083975No Hit
GGAGCCCCACCAGGGAGACCCAGTGACATAGATGGTCTGCTCAGGGTGAT48530.1567052439910013No Hit
GACTGTCATGGGTACCAGTCTCCTATGCTGGGTGGTCCTGGGTTTCCTAG48150.15547820931726175No Hit
GGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC47500.1533793342174441No Hit
GGCAGCTGTGAGGTCTGGTTCCCCGACGTGCTGCAGCAAGTGCCTTTGCC46380.14976281096852753No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC46130.1489555513147515No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG45210.14598483578885574No Hit
GGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTC45120.14569422231349638No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG42880.13846117581566322No Hit
GTCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG42300.13658833341890284No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC41980.13555504106206956No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG41110.13274577746692898No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG40260.13000109464409051No Hit
GGACTCTGTCATGGGCACCAGGCTCCTCTGCTGGGCAGCCCTGTGCCTCC40230.1299042234856374No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG40050.12932299653491866No Hit
ATTCTGTGATCAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCAC39840.1286448984257468No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC39690.1281605426334812No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC39610.12790221954427286No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC39210.12661060409823122No Hit
GGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCATGGG38310.12370446934463755No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT38090.12299408084931465No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT37940.12250972505704905No Hit
TGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCTGGGGCCAG37720.12179933656172615No Hit
ACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGG37100.11979733262036162No Hit
GGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGC36680.11844113640201788No Hit
GGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTG36670.11840884601586686No Hit
GCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC34860.11256428612252845No Hit
GGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT34260.110626862953466No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC34150.11027166870580456No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC33970.10969044175508581No Hit
ACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG33590.10846340708134626No Hit
GATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAG32320.10436252804016408No Hit
GCTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGT32250.10413649533710678No Hit
GTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTC31910.10303862220797139No Hit
GCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCA31800.10268342796030995No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT31160.10061684324664333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)