FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4591412
Sequences flagged as poor quality0
Sequence length116-124
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG3164606.892433090299891No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA2004734.366260313820672No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1638453.5685100792523086No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA1244102.7096239675289433No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT776991.6922680865929696No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC730341.5906653552327694No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC645361.4055806797560315No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT632911.378464838267618No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG522681.1383861870814467No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC494371.0767275949098012No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC468771.020971326467762No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT419800.9143156832800019No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT414190.9020972197659457No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG403530.8788799611100028No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT385610.8398505732005753No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA341880.7446075412095452No Hit
CAGGGCCAGGGTAGTGATGGGGGCTGTGGCTTCTCTATAAGGACATGCCC341240.7432136344984941No Hit
GGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTC339250.73887945581882No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT334080.7276193031686113No Hit
GCACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGA329490.7176223784752925No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG309810.6747597471104749No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG297830.6486675558629894No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT283740.6179798284275076No Hit
GGAGCCCCACCAGGGAGACCCAGTGACATAGATGGTCTGCTCAGGGTGAT279820.6094421498223205No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT266640.5807363834916144No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA260480.5673200313977487No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC256500.5586516740384004No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC251140.5469777053333484No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT239530.5216913664031892No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA222840.48534089295406296No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC212600.4630383855772473No Hit
GGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGGGTGCTGG211410.4604465902863868No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG210440.4583339504274502No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC208010.45304146088392855No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC203780.44382860871557595No Hit
GACAGTCTTGAAGAACGGGTTGAAAACAACAGTGTGCCAAGTCGCTTCTC203590.4434147926607327No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG184380.40157581153684313No Hit
GGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCCATACC173220.3772695632628917No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT171010.3724562291512938No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG170260.3708227447242809No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG158010.34414249908307076No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC153690.3347336287834766No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT149210.32497628180611976No Hit
GACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGT148560.32356059530270864No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG147400.3210341393889287No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT144350.3143913027190764No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC140330.30563582619028745No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG139890.3046775153264399No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG131340.2860557928584932No Hit
CGTCTGGGCGGAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCT129230.281460256670497No Hit
AGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGAC125730.27383732934443694No Hit
GAGAGGTAAGCAGCTGCCCCACTCTGAGAGGGGCTGTGCTGAGAGGCGCT124480.27111485529941554No Hit
TCATAACGCTATGTATTGGTACAAGCAAAGTGCTAAGAAGCCACTGGAGC121600.2648422750996861No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC120130.2616406456227409No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC119680.2606605549665332No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC119490.2602467389116899No Hit
GGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGGGT119170.2595497855561644No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG117820.25660951358754125No Hit
GTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT115540.25164372092942217No Hit
GAGTGCTGGGTAAGCTGGGGCCGCCGGGGGACCGGGGACGAGACTGCGCT113750.24774513809695145No Hit
AGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAG112620.24528402156025206No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG111050.24186459415970513No Hit
GGAGAAGGAAGCCCCCGGCCTGGTCCATACCCCACCACCAACTTGCATAA110750.24121120038889998No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT108620.23657210461618342No Hit
GGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGG107420.23395852953296284No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC104140.22681475763882655No Hit
GGACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGA102020.2221974416584702No Hit
GGAGCTGAGTCTGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCTGGG97880.21318060762135918No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT95490.20797523724727815No Hit
GGCGTCTGGGCGGAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGG93440.203510379813443No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT93040.20263918811903614No Hit
GGCAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGGGTCC90360.19680220376651017No Hit
GGCACAGCCTCGGGGCAGGGCCAGGGTAGTGATGGGGGCTGTGGCTTCTC90090.19621414937278553No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC89260.19440642660689128No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG87760.19113945775286556No Hit
GAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGGGTCCTG86580.18856944225436534No Hit
GGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGA84280.18356009001152587No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT82130.17887743465408898No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC78790.1716029840057917No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC78600.1711891679509484No Hit
GAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCCATACCCCACCA77380.16853203328300748No Hit
ACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGG77090.16790041930456254No Hit
GAGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGT75290.16398005667973165No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT75030.16341378207836718No Hit
GGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGC73640.16038639094030335No Hit
AGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCTG73510.16010325363962108No Hit
GATAACGCTATGTATTGGTACAAGCAAAGTGCTAAGAAGCCACTGGAGCT68980.1502370077004634No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG67900.14788479012556485No Hit
CTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTG67520.14705715801587835No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC66900.1457068108895477No Hit
GAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGA63500.13830168148708938No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG62990.13719091207672063No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT62710.13658107789063584No Hit
GCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCT62470.1360583628739917No Hit
GGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGGGTGCT61000.13285673339704648No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG60580.1319419821179193No Hit
GATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGCC60360.13146282668599552No Hit
GACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGG59200.1289363707722156No Hit
GGAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGT58990.128478995132652No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG57860.12601787859595262No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC57460.12514668690154576No Hit
GGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAAC57450.12512490710918558No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA56300.12262023098776585No Hit
GAGTGAGCCCCACCAGGGAGACCCAGTGACATAGATGGTCTGCTCAGGGT55580.12105208593783351No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC55440.1207471688447911No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC55010.11981063777330374No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG54300.11826427251573154No Hit
GAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCCATACCC53750.11706638393592211No Hit
GAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGGGTGCTGGG53590.11671790725815936No Hit
CGGAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGG53560.11665256788107885No Hit
AGCTCAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCG53060.11556357826307027No Hit
GCAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC52320.11395187362841758No Hit
GAGGTAAGAAGGGGGCTCCAGGTGGGAGAGAGGGTGAGCAGCCCAGCCTG52280.1138647544589769No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC52200.11369051612009552No Hit
CCCCAAGAGTTCTGTGTGTAAGCAGCTTCTGGAAGGAAGGGCCCACACCA52110.11349449798885398No Hit
GGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCC52090.11345093840413364No Hit
GGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGACAGCAGAGCGG51990.11323314048053192No Hit
GGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC51490.11214415086252334No Hit
GAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCC50580.11016218975774773No Hit
GGAGGTAAGCAGCTGCCCCACTCTGAGAGGGGCTGTGCTGAGAGGCGCTG50570.11014040996538754No Hit
CTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGACAGCAGAGCGGGC49280.10733081675092543No Hit
GAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCC48660.1059804696245948No Hit
AGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGG48090.104739021460065No Hit
GACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGAAAACAAC47900.10432520540522175No Hit
AAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGG47470.10338867433373436No Hit
GAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTAC47360.10314909661777248No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC47040.102452143262247No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG46990.10234324430044614No Hit
ATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCCA46740.10179874949144184No Hit
GCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCCAT46680.10166807073728082No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT46510.1012978142671579No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC46390.10103645675883584No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG46210.10064442049635275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAT141550.0118.3156118
CCCATTT30550.0117.87877118
TAGAACG206.050177E-6117.566368
ATAGTTC152.837002E-4117.553562
GATAGTT152.837002E-4117.553561
CTGTATA15950.0116.66716118
GGATCTG65300.0114.493211
GGTTCAC13350.0110.9494251
GCTATTT6850.0110.689121
GACAGTC26050.0110.107741
GAGACTA7100.0109.275131
GAGTGCT14550.0109.070311
GGCGATT650.0108.510981
GCACTCT38000.0107.808981
GCTTCAA5350.0107.665871
GAGGCAT1150.0107.331511
AGTTATA2250.0107.09878118
GGATCCT20300.0106.551021
GAGATCT25950.0106.2285461
GAGAACT8700.0106.0684361
Produced by FastQC (version 0.11.5)