FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4591412
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT50172410.927444542114713No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1380033.005676685080755No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC1272022.770433147798542No Hit
CCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGG1127322.45527955234686No Hit
CTCAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCA894141.9474183540923795No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC854341.8607347804988967No Hit
CTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGC798351.7387897230742961No Hit
TGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGT699981.5245419056272884No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA647711.4106989309606717No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG558981.2174468333488695No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG460751.0035039329949045No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG387680.8443589902191309No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA343260.7476131525552488No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC277470.6043238986176801No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT266020.5793860363652837No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC262730.5722204846787873No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC250780.5461936328083823No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC249350.5430791225008778No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC231620.5044635506462936No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG223150.48601606651722823No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC214760.4677428207270443No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA170630.3716285970416072No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA170300.37090986389372155No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA162920.35483637713191496No Hit
TAACCTCTGCCACCTGCGCTTCCTGCCGCTGCCCAGTGGTTGGGGGAGGG159320.3469956518822532No Hit
AGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG156540.3409408696061255No Hit
GGGGGCTGTGGCTTCTCTATAAGGACATGCCCCAACGTGACAACAGCTTG152580.3323160718314976No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA146770.31966201247023796No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG145000.31580698922248757No Hit
GGCTGGGAGAGCGAGGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT141500.3081840618964275No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT135380.2948548289720025No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG132570.2887347073187943No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA123570.2691328941946399No Hit
AGTTGGTGCTGGTATCACTGACCAAGGAGAAGTCCCCAATGGCTACAATG115970.25258025200090956No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA114890.250228034426011No Hit
CCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGG109470.238423386966798No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC108310.23589693105301812No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA104690.22801264621863604No Hit
CTGCACGACCCCAGAACCCTGTTCTTAGGGGAGTGGACACTGGGCAATCC103320.2250288146652925No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG101980.2221103224890295No Hit
CACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGG101230.22047683806201668No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT100620.21914827072804619No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC98350.21420425786228725No Hit
GGCTGGGAGAGCGATGGGGCGCTCAGCGGTGGGAAGGACCCGAGCTGAGT96080.20926024499652832No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG95270.2074960818153544No Hit
GGCGGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT92100.20059188763718006No Hit
AACTTATTCAGAAAGCAGAAATAATCAATGAGCGATTTTTAGCCCAATGC90130.19630126854222624No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT88680.19314319865000135No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC87250.1900286883424968No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG86160.18765469097523813No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG85460.1861301055100261No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC84570.18419170398997084No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC83070.1809247351359451No Hit
GCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACA82740.18020600198805944No Hit
AAACCTCTGCCACCTGCGCTTCCTGCCGCTGCCCAGTGGTTGGGGGAGGG79080.17223459798423665No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC78200.17031797625654155No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA75050.16345734166308754No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG73430.15992901530073972No Hit
GCTCTCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA72790.15853510858968875No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG68820.14988853102270064No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA68460.14910445849773446No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA67530.14707893780823852No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG66860.14561969172010703No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGGGGGAAGGACCCGAGCTGAGT66820.14553257255066634No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA65370.14237450265844145No Hit
TCCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTG63860.13908575401205556No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA61970.13496937325598313No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA60970.13279139401996598No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC59940.1305480754068683No Hit
GAGTGTGTGGTGGGGCCCCAAGAGTTCTGTGTGTAAGCAGCTTCTGGAAG59030.1285661143020927No Hit
CAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTGCCTGGAGATCCGTC58630.12769492260768583No Hit
TGGGGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT58500.1274117853070036No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT55870.12168369991627849No Hit
GGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGT54650.11902656524833755No Hit
TACCATAACGGAGGCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACC54200.11804647459212983No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC54190.11802469479976967No Hit
TTGGGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT53270.11602095390263387No Hit
GGGGGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT52240.1137776352895362No Hit
CCCTCCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCT51550.11227482961668436No Hit
GCTCAAAACATCCTGAGGACAGTGCCTGGAGGTTCTTTTGCAGATCCGTC51380.11190457314656146No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT50190.10931277785570102No Hit
AAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGACCCA49780.108419806368934No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA49720.10828912761477298No Hit
CCCTCAAAGGAGCAGCTGCTCTGGTGGGCTCTCCCAGGCTCTGGGGGCGG49710.10826734782241279No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC48990.10669920277248046No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC48950.10661208360303975No Hit
TCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGA48570.10578445149335325No Hit
GTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTG48300.10519639709962861No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC48170.10491325979894639No Hit
CCCTCAACGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGG46090.10038306298803071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)