FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5830730
Sequences flagged as poor quality0
Sequence length109-122
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT3572916.127723286792563No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT2049873.5156318334067946No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG1159441.9884988672087371No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT920041.5779156297753454No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC722391.238935776480818No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG594121.0189461696905877No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG581900.9979882450396435No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG519600.891140560444404No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC465710.7987164557439634No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA447290.7671252141670083No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT372150.6382562732282235No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA363960.6242100045791865No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG352730.6049499805341698No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT311860.5348558413783523No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT240860.41308721206435556No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG222860.38221629195658174No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA221410.3797294678367889No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC215720.36997082698049816No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG213100.3654773930537No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC211240.36228739797589665No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG208540.3576567599597306No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC205050.3516712315610567No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT194370.33335448563044423No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT193900.33254841160540793No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT190880.3273689572317703No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC187450.32148633190012227No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA183460.3146432779428991No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG178270.30574216264515763No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT164500.28212590876271065No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT159800.2740651685123475No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG155700.2670334589322435No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG154610.26516405321460607No Hit
ATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCTG151480.25979594321808763No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT150380.25790938698927923No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC149940.25715476449775587No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC139560.23935253390227296No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC135740.23280103863495655No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT133400.2287878190209459No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT131370.22530626525323585No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC130140.22319675237920467No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT124780.21400407839155647No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC123520.21184311398401232No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT122080.20937344037539038No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG116210.19930609031802193No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC115750.1985171668041566No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC113880.19531002121518232No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC111820.1917770159139593No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC111100.19054217910964838No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT110840.19009626581920272No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC110820.19006196479686077No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC105900.18162391330073593No Hit
GGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC105390.18074923723101566No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG104940.17997746422832134No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT103440.17740488755267353No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC102700.17613574972602059No Hit
GAGAATGACTTCCTTGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATA99650.17090484381887003No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA98790.16942989985816528No Hit
GGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC93770.1608203432503306No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCC92920.1593625498007968No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG88040.15099310034935592No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG86960.14914084514288947No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC84790.14541918421878564No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC83010.14236639323035025No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC82390.14130306153774913No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG82300.14114870693721027No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA81230.13931360224191483No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA80860.13867903332858836No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG80150.13746134703544838No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC79870.1369811327226608No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC78950.13540328569493015No Hit
GCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCAC77710.13327662230972792No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT75700.12982936956435986No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG72060.12358658349812116No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG71190.12209448902624544No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA70880.12156282317994488No Hit
CAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG70150.12031083586446295No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG70030.12010502973041112No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG69130.11856148372502243No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA68120.1168292820967529No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT65540.11240445021463866No Hit
GGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAG64570.11074085063105307No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC63960.10969466944962296No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT63460.10883714389107368No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA62950.10796246782135341No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT62340.1069162866399233No Hit
GGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCC60660.10403500076319776No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC60500.10376059258446198No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG60200.10324607724933241No Hit
GAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG59990.10288591651474173No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC59560.10214844453438936No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT58990.10117086539764318No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC58370.10010753370504206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)