FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403405
Sequences flagged as poor quality0
Sequence length112-122
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT162624.031184541589718No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT150903.7406576517395673No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT112772.7954537003755533No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT109272.7086922571609175No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT108212.682415934358771No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC82172.0369107968418834No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC60781.506674433881583No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG46021.1407890333535777No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA41401.0262639283102588No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT36120.8953780939750374No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT36060.8938907549485009No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC35110.8703412203616713No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT34570.8569551691228419No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT31860.7897770230909383No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC29710.7364807079733766No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT27810.6893816387997174No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC27200.6742603586965952No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT24770.6140231281218627No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG21190.5252785662051784No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC19380.4804105055713241No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG19100.47346959011415324No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG18620.46157087790186047No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC18360.4551257421202018No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG17950.44496225877220164No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA17750.44000446201707966No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT17430.43207198720888434No Hit
GCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTG17050.42265217337415256No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT16920.4194296054833232No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT16580.4110013509996157No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG16480.4085224526220547No Hit
GAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTG16330.4048041050557133No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC15850.39290539284342035No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA15520.38472502819746907No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA14680.3639022818259566No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG13520.3351470606462488No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG13180.3267188061625414No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG12700.3148200939502485No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT12680.3143243142747363No Hit
AAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCCT12470.30911862768185816No Hit
GAAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTC12400.3073833988175655No Hit
GGCAGATGCAAATCAAGAGCCAACCATGCAAGCAGTCCCTTCTAAGGCCA12260.3039129410889801No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG11750.2912705593634189No Hit
CTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTT11640.2885437711481018No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC11560.286560652446053No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG11010.2729267113694674No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC11000.2726788215317113No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC10980.2721830418561991No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG10880.2697041434786381No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC10790.2674731349388332No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC10410.25805332110410134No Hit
GACAGACCATCAACCCACTGCCTGGTCCTGGGAGAAGACCTATTCTTTCT10260.2543349735377598No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG10130.2511124056469305No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC9780.24243626132546695No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC9690.24020525278566204No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG9550.23673479505707662No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC9360.23202488813971073No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC8880.22012617592741787No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC8720.21615993852332022No Hit
GTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTG8470.20996269257941771No Hit
GCTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTT8290.20550067549980786No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG8230.20401333647327127No Hit
ATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCTG7980.19781609052936874No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT7620.1888920563701491No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC7470.1851737088038076No Hit
ATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACTG7410.18368636977727099No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC7370.18269481042624658No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT7310.18120747139970997No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT7290.18071169172419776No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT7270.18021591204868556No Hit
GGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGG6800.1685650896741488No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG6770.1678214201608805No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG6690.1658383014588317No Hit
GACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTC6650.16484674210780728No Hit
GGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC6630.16435096243229508No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG6510.16137628437922188No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT6360.15765793681288034No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC6340.15716215713736814No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG6270.15542692827307544No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT6230.15443536892205104No Hit
GATTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTC6100.15121280103122173No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT6030.149477572166929No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC5930.146998673789368No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA5910.1465028941138558No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC5750.14253665670975818No Hit
GAGAGCCTGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCT5750.14253665670975818No Hit
GGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC5740.1422887668720021No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT5730.142040877034246No Hit
GGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGT5720.1417929871964899No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT5680.14080142784546548No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT5650.14005775833219716No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC5620.13931408881892887No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCGTTTGT5420.13435629206380684No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT5310.13162950384848973No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC5230.12964638514644092No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC5140.12741537660663602No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG5120.1269195969311238No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG5120.1269195969311238No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT5030.12468858839131891No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC5010.12419280871580671No Hit
ATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCC4850.1202265713117091No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT4840.11997868147395298No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC4820.11948290179844077No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT4810.11923501196068467No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC4790.11873923228517247No Hit
GGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT4750.11774767293414806No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC4740.11749978309639195No Hit
TCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT4580.11353354569229435No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT4550.11278987617902604No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA4520.11204620666575772No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT4510.11179831682800163No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT4440.11006308796370894No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC4400.10907152861268452No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC4320.1070884099106357No Hit
ATATGATGTTAAAATGAAAGAAAAAGGAGATATTCCTGAGGGGTACAGTG4130.1023785029932698No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC4080.10113905380448927No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC4070.10089116396673319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGTT356.91216E-11115.804541
GGTTCAC1050.0115.804541
GAGATAC356.91216E-11115.804541
GAAGCTA401.8189894E-12115.8045351
TAGGTAT153.0093745E-4115.8045357
GCATTCC206.5145523E-6115.8045351
GGTATGT153.0093745E-4115.8045359
TCGTATC153.0093745E-4115.8045354
GGTGCCA153.0093745E-4115.8045351
GCCAGAG206.5145523E-6115.8045351
GGATTGG153.0093745E-4115.8045351
ACGACAA950.0109.419174116
GCTTCAA13900.0108.722971
GAGGAGC700.0107.532791
GAGAACT2550.0106.7218251
GATGTTC17800.0106.696311
CAGGTTT750.0105.98641116
GCTATTC2300.0105.734571
GAGAGCT4350.0105.1558461
GTCTTTC5350.0103.89941
Produced by FastQC (version 0.11.5)