FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10106469
Sequences flagged as poor quality0
Sequence length110-123
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG3450443.414090519646377No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC3091813.0592385926281476No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG2503142.476770076670695No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT2471542.4455029743820518No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG2440032.414324923967016No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC2115892.0935996538454726No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG1769711.7510665693428635No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC1750231.731791786033282No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA874560.8653467397960652No Hit
GAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG801430.7929871451641517No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT801040.7926012537118552No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA782590.7743456196224417No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT773080.76493580497798No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC769040.7609383653182927No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG623090.6165259102857783No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATG618080.6115686893216612No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG590080.583863661977294No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC540230.5345388186516973No Hit
GCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCG520780.515293719299985No Hit
GAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCC503880.49857175636713474No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT480740.47567553019753983No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT470330.46537519681700895No Hit
GAGACATCTGTGTACTTCTGTGCCAGCAGTACCGGGCCACGTAGCGGGGC436280.43168390463573386No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT423920.4194541139937203No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT418770.4143583678928813No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA407240.40294983341857576No Hit
ACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGC407010.40272225640824705No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC389490.38538682501277155No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT383880.3798359248912751No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC365260.36141208170727085No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC354040.35031028146427795No Hit
GAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATA344320.3406926791147334No Hit
GGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAAT342160.33855543414816786No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA339500.33592345655045297No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC334420.33089697301797494No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC318770.31541184166299824No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT291450.28837965069699417No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC260430.25768643826048443No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC244380.2418055208005882No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT242190.23963859187615377No Hit
GACACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC233670.23120834784136776No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA207850.20566035476881195No Hit
GACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCCTCCT206760.20458183763290622No Hit
GGAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCA206550.20437404992782346No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT199680.1975764235758305No Hit
GACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGA189360.18736514206890656No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG188970.18697925061661003No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC187990.18600957465955717No Hit
GGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGT187460.18548515807053878No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG182760.1808346713377343No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC181660.17974625954920556No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT181580.17966710232822167No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT175900.17404693963836432No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG174940.17309705298655742No Hit
GTCAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG172070.17025728768375978No Hit
GGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT165850.16410281375226105No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG164880.16314303244783118No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG164260.1625295639852059No Hit
ATGAATACATGTCCTGGTATCGACAAGACCCAGGCATGGGGCTGAGGCTG158730.15705782108469338No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT152320.15071534875335788No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA150870.1492806241230246No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG148560.14699495936711426No Hit
GCTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGC146540.14499623953727064No Hit
GAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAATG144920.14339330581234652No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT143100.14159247903496264No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT141140.13965312712085695No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG138360.1369024136916662No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC137870.13641757571313978No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC135250.13382517672591684No Hit
AATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCT134860.13343928527362028No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA134250.132835711463618No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC133590.13218266439050078No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC131790.13040162691836288No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC131280.12989699963459048No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT131200.12981784241360658No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG130600.12922416325622726No Hit
GGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATG129100.12773996536277904No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT129080.12772017605753305No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC126420.12508819845981817No Hit
ACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGG125420.12409873319751932No Hit
GGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAA123750.12244632620948027No Hit
GACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCC123560.12225832780964352No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC122480.12118970532636077No Hit
TGAGGCTGATTCATTACTCAGTTGGTGCTGGTATCACTGACCAAGGAGAA121890.12060592082160447No Hit
ACATCAACCCTGGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGT121340.12006171492734011No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG121260.1199825577063562No Hit
GCTTGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCC118590.1173406854560183No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG116310.11508470465797697No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG112810.11162157623993109No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG112620.1114335778400943No Hit
AGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTC111570.11039463931468052No Hit
AGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGT110610.10944475266287364No Hit
TCAACTAGAAAAATCAAGGCTGCTCAGTGATCGGTTCTCTGCAGAGAGGC110170.10900938794746216No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT107890.10675340714942083No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG106680.10555615418203923No Hit
GAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTC105820.10470521405646226No Hit
AGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGAC105550.10443805843564156No Hit
GACACCAGCTCCTCTGGGGTTTGCCACACTCATGATGCACTGTGTAGCAA105210.10410164024645997No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG103750.10265702096350367No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG102600.10151913591186003No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC101100.10003493801841176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGT282300.099.474321
GCTTTCC45950.097.872931
GAGATAC71500.096.3903051
GTCTTTC19300.096.23371
GAGGAGC40350.095.968211
GATCTCA63150.092.58021
GAGTCAG78250.091.285011
GAATGCA110200.090.523991
GAGAACT22550.090.13431
GGCACAG269650.089.758731
GCTTTTC57550.089.4103851
GACAGAC468400.088.955661
AGTCATT23050.088.690284
GACTCAT26050.088.337661
TGCTTAC28350.087.566586
GAGGAGG267350.086.554891
GTATGCA13100.086.494371
GGATCTG59100.086.274841
TCTACGC5300.085.980669
GACAGAT530450.085.6188051
Produced by FastQC (version 0.11.5)