FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10106469
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA160560715.886923514038385No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT6735576.664612536782134No Hit
CCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAG3133833.100815922949944No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG2192722.1696202699478917No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1909301.889186025307157No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA1711191.693163062193136No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA1631411.6142235235669353No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA640510.633762395155024No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG528320.5227542873777181No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT520040.5145615150058839No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC462820.4579443126971448No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG385600.3815378051424291No Hit
CGATCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGA374440.37049537281517414No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC372090.36817012944877187No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA370080.36618130427155127No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA359960.35616791581708707No Hit
AACCACAGAGGAGTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA339420.3358442993294691No Hit
CACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA326740.3232978798035199No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG326420.3229812509195843No Hit
AACCACAGAGGATTTCCCGCTCAGGCTGCTGGCGGCTGCTCCCTCCCAGA323180.31977538346973605No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC306980.303746046220495No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT303290.30009491940261235No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC302530.2993429258032652No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG301830.29865030011965604No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG280280.2773273237171162No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA268110.26528553147493944No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA262330.25956642225885224No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC254360.25168038411833055No Hit
TCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCT240630.2380950260669676No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC236920.23442410994383894No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC225760.223381677616584No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA198660.19656716900828566No Hit
ACCCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA194290.1922432058120398No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT193090.1910558474972812No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT189340.18734535276366057No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA187100.1851289505761112Illumina RNA PCR Primer (96% over 28bp)
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG185460.18350622754594112No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC183660.18172519007380322No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC179920.1780245899928056No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG174220.17238463799770226No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG172370.17055412726244942No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA171700.1698911855367092No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG171560.16975266039998738No Hit
AGTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAG169650.1678627817489966No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC169450.16766488869653684No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA156490.154841418897144No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC155510.15387174294009115No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA155080.15344627287730264No Hit
CTCAGGGCGCCAGTGCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT154940.1533077477405808No Hit
AACCACAGAGGATTTCCCGCGCAGGCTGCTGTCGGCTGCTCCCTCCCAGA153720.15210060012057625No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA146680.14513476467399247No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC143200.14169142556119252No Hit
TTTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTG142290.1407910121725006Illumina RNA PCR Primer (96% over 30bp)
AACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCGCCCTCCCAGA139120.1376544072910133No Hit
GCCAAGTCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTC136600.13516095483002025No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA136550.1351114815669053No Hit
GTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTG133510.13210350716951688No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG132610.13121298843344792No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG131140.12975847449786865No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC130450.12907574346688244No Hit
AACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCGGCTCCCTCCCAGA126830.1254938792173607No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCGCTGAGATGAATGTGAGCACCT125800.12447472999719289No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC121760.12047729033750562No Hit
AACCACAGAGGATTTCCCGCTCAGGCGGCTGTCGGCTGCTCCCTCCCAGA119630.11836972932880911No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC118530.11728131754028039No Hit
CTCAGGGCGCCAGGTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT118200.11695479400372177No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT118090.11684595282486891No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG115020.11380829446961148No Hit
ATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGA111370.11019674626222076Illumina RNA PCR Primer (96% over 26bp)
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG106220.10510100016138178No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA102860.10177639688005771No Hit
AACCACAGAGGATGTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA101960.10088587814398876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)