FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5630757
Sequences flagged as poor quality0
Sequence length116-124
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT3863046.8606050660683815No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG1745203.0994056394193534No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG1446082.5681804418127085No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT1258412.23488600200648No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG1240392.2028832002517604No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT1161692.0631151370943552No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT898221.5952029185418586No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT860851.5288352880438634No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT790631.4041273668886796No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA679321.206445243508111No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC668901.1879397388308535No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT534120.9485758309229115No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC464310.8245960534258536No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC417330.7411614459654359No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC395430.7022679188606434No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC393260.6984140853529996No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT392580.6972064324565951No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT378260.6717746832264294No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG362280.6433948401609233No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC351300.6238947978042739No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT319520.5674547844987806No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC305770.5430353325494245No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG299890.532592686915809No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG285820.5076049277210862No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA284690.5055980927608845No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG275380.4890639038409933No Hit
GAGAGTATAGCTGGTTCCCAGGGAGGCTGGGGGTGATTCACCACACTCTT268840.4774491245138087No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG267260.47464310748981No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT267100.47435895386712656No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC247600.43972773110258534No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG238330.4232645805883649No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC234910.4171907969035069No Hit
GCTATTTCCCCAGGCAGGGCTGGGAGAGATGAGATCCTGGCCTGGACCTG228870.40646399764720803No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT226510.4022727317126276No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG222390.3949557759285297No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG200670.3563819216492561No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA194350.3451578535532611No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG193120.34297342257888236No Hit
GGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC190060.33753898454506204No Hit
GGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATG187670.3332944398062285No Hit
ACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGG181990.32320698620096733No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC181650.32260315975276505No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC170000.30191322410112886No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA169820.30159355127561No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC167980.29832578461475073No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG167170.29688725689991596No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT167100.29676293968999196No Hit
GAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATGCACAGACC165800.2944541915056892No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT162720.28898423426903347No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG153020.2717574208938514No Hit
GCCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCC152460.2707628832144594No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC150220.26678473249689166No Hit
GGATTCACCACACTCTTAAAAGAAGACTAGCCTCATTTCCTCCCCAGCTC149380.2652929259778037No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC146490.26016040116808453No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC144800.25715902852849093No Hit
GGAATGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACC141110.2506057356053547No Hit
GGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGC140750.24996638995431697No Hit
GAATGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCA138050.24517129757153436No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT137270.24378604866095271No Hit
AGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCTG135450.24055380120292885No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC133150.23646909287685475No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT131720.23392946987412172No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC131640.23378739306277999No Hit
GAGGGAGGCTGGGGGTGATTCACCACACTCTTAAAAGAAGACTAGCCTCA130070.230999135640199No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC126970.22549365920070782No Hit
GAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGGC125990.2237532182617719No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC124720.22149774888172233No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT124460.22103599924486175No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG122230.21707560812871166No Hit
GACACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC121610.21597451284081343No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC119880.212902101795549No Hit
GGAAAGACGTGTTCCCTTTTCACCAATGCACAGACCCAGAGGACCCCTCC116790.20741438495747552No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG116350.2066329624950961No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT113610.20176683170664264No Hit
GAGAATGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCAC112110.19910289149398563No Hit
GCCAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAG111610.19821491142309997No Hit
AGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGGA111340.1977354021848217No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC110790.19675862410684747No Hit
GATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGCC110610.19643895128132863No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC110250.1957996056302909No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT108660.19297582900487448No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG108400.19251407936801393No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC105540.18743483336254788No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG98580.1750741507758193No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT98040.1741151322992628No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT97140.17251676817166856No Hit
GGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTG92640.1645249475336975No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT88730.15758094337937154No Hit
GAGCAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGA88490.15715471294534641No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC88480.15713695334392871No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT88320.1568527997212453No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT84970.1509033332463113No Hit
GACAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGT84390.14987327636408393No Hit
GATTCCAGCCTCTGACAAACTCTGCACATTAGGCCAGGAGAAGCCCCCGA84050.14926944991588165No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA83310.14795523941097086No Hit
GACTGAGAACCTAAGTTCTATTTCCCCAGGCAGGGCTGGGAGAGATGAGA82330.14621479847203495No Hit
GAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGGGTCCTG81390.14454539593876986No Hit
GGAGCCCCACCAGGGAGACCCAGTGACATAGATGGTCTGCTCAGGGTGAT81010.14387053108489675No Hit
TTCCCAGGGAGGCTGGGGGTGATTCACCACACTCTTAAAAGAAGACTAGC78910.14014101478717694No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC78880.1400877359829238No Hit
GTCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG77830.13822297783406387No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG77770.1381164202255576No Hit
GGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATGCAC77020.13678445011922907No Hit
GGCAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGGGTCC76910.13658909450363424No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG75590.13424482711649605No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT75220.13358772186404066No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC74400.13213143454778817No Hit
ACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATG72860.12939645592946028No Hit
GCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCT71860.12762049578768894No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC71780.12747841897634724No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC71440.12687459252814498No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT69720.12381994108429827No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT68880.12232813456521031No Hit
GAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATGC67830.12046337641635041No Hit
GAGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCT67760.1203390592064264No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC67520.11991282877240131No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC65710.11669834091579516No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT63730.1131819398350879No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT63330.11247155577837936No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT63080.11202756574293651No Hit
GGACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCA61820.10978985596430463No Hit
GAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTA61680.10954122154445663No Hit
GAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCC61510.1092393083203555No Hit
GCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCT61470.10916826991468466No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC61470.10916826991468466No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG61310.10888411629200125No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT61190.10867100107498867No Hit
GGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCC59900.10638001249210363No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA59840.10627345488359735No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG59790.1061846568765088No Hit
GGAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGG59270.10526115760278769No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA59030.10483492716876255No Hit
GAGCTGTAGCATCAGCTTTGTCCTTCTCTGCAGGTCCAGTGAATGCTGGT58690.1042311007205603No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA58450.10380487028653518No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG58140.10325432264258606No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT57310.10178027572491585No Hit
GACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCT56480.10030622880724564No Hit
GGCAGAGTATAGCTGGTTCCCAGGGAGGCTGGGGGTGATTCACCACACTC56400.10016415199590394No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG56400.10016415199590394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCCG21700.0117.53712118
GGATCTG100050.0114.940931
GACTGCA6100.0112.997291
GAATGCG28850.0110.6790541
GAGAACT26900.0110.379961
GACTGTA2350.0110.3054051
GCTATTT24900.0109.5452651
GCTTCAA26750.0109.457261
GACTACG10400.0109.329141
GAGAGAC16550.0108.215031
GAGATCT81200.0107.015821
GACAGAC202400.0106.736361
GATGTTC98950.0106.4544141
GGTTCAC31150.0105.532971
GCACTAC6200.0105.473481
GAGTCAG59550.0104.6684651
GGTCACT6800.0103.0979461
GAGTGAT15150.0103.049341
TTGTCCG302.9095827E-7102.67936118
GAGAGTT15900.0101.523231
Produced by FastQC (version 0.11.5)