FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706334
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA1310077.677687955581967No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT994275.8269365786534175No Hit
CCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAG557283.265949104923186No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG422632.4768304446843348No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA322871.8921852345437646No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA304401.7839414792180195No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG282231.654013809723067No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT262261.5369792783827785No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA190931.1189485763045217No Hit
CGATCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGA104990.6152957158446118No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG98170.5753269875651543No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA96450.5652468977351445No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC87110.5105096657512538No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA82630.48425454805448404No Hit
TCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCT81680.4786870565786065No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG81000.47470190478534685No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC72380.42418424528843707No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT71050.41638975722220856No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG70310.41205297438836713No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC69580.40777479672795597No Hit
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG67170.39365094993125616No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC66880.39195139990177774No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA63860.3742526375258302No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT62470.36610651841902No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG61860.3625316028397723No Hit
AGTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAG56330.3301229419328221No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA49200.2883374532770255No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA49160.28810303258330433No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC48710.28546579977894126No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC48470.28405927561661437No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC48340.28329740836202055No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG46030.26975961329962367No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC45760.26817727361700583No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA42960.2517678250565247No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT42590.24959943363960396No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC41760.24473520424488993No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT41120.24098447314535137No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG39770.23307277473226226No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG38590.22615736426748806No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC37530.21994521588387736No Hit
CAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGGAGTCTG36380.21320562093939405No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC36140.2117990967770671No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT35400.20746231394322565No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC35350.2071692880760742No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG32300.18929471017983585No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC32070.18794679119093916No Hit
CTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGC30490.1786871737889534No Hit
ATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG29660.17382294439423934No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT29610.1735299185270879No Hit
AACCACAGAGGAGTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA29310.1717717633241792No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG28740.1684312684386527No Hit
ATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGC28560.1673763753169075Illumina RNA PCR Primer (96% over 29bp)
CACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA27490.16110562175986648No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA27170.1592302562100972No Hit
AACCACAGAGGATTTCCCGCTCAGGCTGCTGGCGGCTGCTCCCTCCCAGA26990.15817536308835198No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG26580.1557725509777101No Hit
AGCTGAAGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCA26110.15301810782648648No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA25540.14967761294096No Hit
TGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG25040.1467473542694455No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC24860.1456924611477003No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC24600.14416872663851274No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG24410.14305522834333723No Hit
GTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTG24390.14293801799647668No Hit
GCCAAGTCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTC24320.14252778178246464No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC24120.14135567831385884No Hit
TAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGC24060.1410040472732771No Hit
GAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGG24000.14065241623269537No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT23790.13942170759065928No Hit
CTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCC23720.13901147137664724No Hit
TTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTC23630.13848402481577463No Hit
CTCAGGGCGCCAGTGCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT23030.13496771440995725No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA22420.1313927988307096No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG22040.12916580224035856No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG21810.1278178832514619No Hit
GTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGT21030.12324667972389931No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT20430.11973036931808193No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA20160.11814802963546411No Hit
TGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCACAG19920.11674150547313715No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC19490.11422148301563467No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT18820.11029493639580527No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC18560.10877120188661775No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCGCTGAGATGAATGTGAGCACCT18490.10836096567260571No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA18390.10777491393830281No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC18160.10642699494940616No Hit
ACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA17910.10496186561364891No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG17890.10484465526678832No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG17770.10414139318562486No Hit
TGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAA17650.10343813110446137No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC17530.1027348690232979No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA17270.10121113451411036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA279500.0144.7246145
GGTCACA397400.0144.16614145
GGTGCTC302250.0143.3736145
CGTTGTA67950.0142.58578145
AGAGCGA41000.0142.191598
GCGATTC46350.0141.909071
CATCCTA75550.0139.46469145
AGTTGTA89650.0138.8696145
TGTTGTA20700.0137.58038145
GAGAGCG42350.0137.495767
GAGCGAT42150.0136.419779
CGTACTG50200.0135.4046145
TTGACCA18500.0135.13931145
AGTGCTC222200.0134.1045145
CTTTCCA5850.0133.78339145
GTAAAGC2900.0130.034321
GGGTTGC15700.0129.780179
GCTGGGA41750.0127.317292
CGATCGG14700.0127.278481
TGGGAGA42250.0127.179954
Produced by FastQC (version 0.11.5)