FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4587342
Sequences flagged as poor quality0
Sequence length110-123
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT2330095.079390200251039No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1474303.213843659356551No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA1242492.7085183533296626No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT938332.045476443657351No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC627391.3676547333946325No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA551291.2017634612810644No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT532611.1610427127517415No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC526091.146829689175126No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT417610.9103528797286098No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG406650.8864610486857095No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC344020.7499331857097203No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG294150.6412209946413413No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC262860.5730115609431344No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT256370.5588639347142637No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG231510.5046713325494372No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC228230.49752122252929915No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG204860.4465766886358157No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT195930.4271100781236716No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT195420.42599832321200376No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT192990.4207011380446455No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC185020.4033272426603467No Hit
GACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTC175480.3825308860773842No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT174110.37954440719702176No Hit
GGCAGATGCAAATCAAGAGCCAACCATGCAAGCAGTCCCTTCTAAGGCCA172730.37653612920074414No Hit
ATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACTG164460.3585082603389937No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG162610.35447542389470854No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT162430.3540830398082375No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA160490.3498540113207169No Hit
GAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGAGGACCTGAAAAA155510.3389980515950195No Hit
GGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGG154350.33646935414887313No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG148690.32413105454095203No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC146290.31889926672133884No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC135680.29577040473546556No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG129260.28177537231800026No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG124700.2718349754607352No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA124070.27046163115808675No Hit
GATTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTC119320.2601070510984356No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC117990.25720776868173334No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC117910.25703337575441293No Hit
GGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGT116520.25400329864222027No Hit
ATATGATGTTAAAATGAAAGAAAAAGGAGATATTCCTGAGGGGTACAGTG112430.2450874602329628No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA111460.24297294598920244No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC111250.2425151645549863No Hit
GGACAGAGGTGGAGAGAGCCACACAGATAGCCAGCTGCCTGTGCTGCCTG107840.23508166602795258No Hit
GACTATAGTGGGAACACTCCCAAAATTCAAGTTCGCAGATGCAAATCAAG104370.2275173728054285No Hit
GACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGA104150.2270377922552973No Hit
GAGAGCCTGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCT102880.2242693045340853No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATCTG100210.21844894058476566No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC98380.21445970237231057No Hit
GCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTG94820.20669921710655104No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC94130.20519507810841223No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT90310.19686781582886123No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT89320.1947097033532708No Hit
AGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTG85720.18686202162385102No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA80260.17495970433423103No Hit
GAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTG79890.174153137045374No Hit
GAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGG78900.17199502456978355No Hit
GACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGT76510.1667850358660854No Hit
AGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAG76270.1662618570841241No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC74800.163057387044611No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG72200.15738961690669673No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACC71150.15510070973561596No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG69900.1523758202462341No Hit
GAGTGCTGGGTAAGCTGGGGCCGCCGGGGGACCGGGGACGAGACTGCGCT69210.1508716812480953No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG68840.15006511395923827No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC66630.1452475093420111No Hit
GGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTC65450.14267521366403466No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCGTTTGT63520.13846798429242904No Hit
GAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGAGGACCTGAACAA62590.13644066651232892No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCA62480.13620087623726332No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC61720.13454414342771914No Hit
GGAGATTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCT61390.13382477260252235No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC60820.1325822229953642No Hit
GACACAGATAGCCAGCTGCCTGTGCTGCCTGCTCTTCCCCTAATTCTGCC59080.12878917682614463No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT58970.12854938655107903No Hit
AGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGAC58230.12693625197336497No Hit
GCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACTGGA57220.12473454126594441No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC56980.1242113624839831No Hit
GGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC56070.1222276429357131No Hit
AGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGCT54120.11797681533227738No Hit
CTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTT53430.1164726763341386No Hit
GTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT52780.11505573379966003No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC52580.11461975148135892No Hit
GGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCT52250.1139003806561621No Hit
GACACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC52170.11372598772884167No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGG51690.11267963016491903No Hit
GAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAA51050.11128448674635552No Hit
GGAGCTGAGTCTGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCTGGG50030.1090609769230199No Hit
CAGGGCCAGGGTAGTGATGGGGGCTGTGGCTTCTCTATAAGGACATGCCC49530.10797102112726717No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC49100.1070336591429198No Hit
GAACGACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAGAGG48850.10648868124504343No Hit
GGCCTGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCC48640.10603089981082728No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG48580.10590010511533694No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT48220.10511533694239497No Hit
GAGAGGTGGAGAGAGCCACACAGATAGCCAGCTGCCTGTGCTGCCTGCTC48020.10467935462409386No Hit
GACAGAGAAGCCGCCGACAGAGGTGGAGAGAGCCACACAGATAGCCAGCT47950.1045267608126885No Hit
ACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGG47610.10378559087157661No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTACCAATCTG47430.10339320678510562No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG46930.10230325098935288No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC46900.10223785364160771No Hit
GGATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCAC46900.10223785364160771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCT309850.0104.016921
GACTATA13650.0103.885291
GAATGCG20300.0103.774731
GATGTTC119300.0101.8405461
GCTTCAA84000.0101.0102841
GGATCTG31550.096.549841
GTCTTTC39900.094.3337861
GCCTGTA8050.094.238411
GAGAGCT92900.093.971541
GAGAACT21000.093.3685151
AGTGGTA247400.093.1949842
AGTCATT12150.093.1804664
GAGTGCT9000.092.071731
GACTCAT15500.091.8625641
GATGCTC38250.091.080061
ATTGAGG11150.091.075638
GAGATGC89300.091.029071
CATTGAG11350.091.014367
GAACGAC6750.090.774941
GAGCTTG78600.089.686041
Produced by FastQC (version 0.11.5)