FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4587342
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC1840314.011713100963477No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA1556283.3925528116281716No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA1423643.1034093381308825No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1071242.3352084932843464No Hit
GCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAGGCCTGGGGG1063812.3190117501594605No Hit
AAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC989612.1572623100697528No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG715641.5600319313449924No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC671651.4641376204346657No Hit
TGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGT563601.2285981729724968No Hit
AGCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTACGA425690.9279665653879742No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG359470.7836128197984803No Hit
TGTCACCCACCCTCCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTC338740.7384232525065714No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT311510.6790642598698767No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT279410.6090890977825504No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT275870.6013722107486209No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA261000.5689569253829342No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC228500.49810979865900556No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC226730.4942513551420408No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG220390.480430715651896No Hit
TCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTC215020.4687245904055115No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG209100.4558195137837989No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA204180.44509434875359194No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA203760.4441787858851597No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT198390.43247266063877515No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC159990.3487640555249641No Hit
AAGCGATTCTCATCTCAATGCCCCAAGAACGCACCCTGCAGCCTGGCAAT158800.3461699607310726No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG156240.3405893870568185No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT141820.3091550619073093No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA141160.30771632025691564No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC136810.29823370483386674No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC121780.2654696336135392No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC121510.26488105748383267No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG120750.2632243246742885No Hit
GGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC116060.2530005393101277No Hit
TTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTAT103590.22581704176405423Illumina RNA PCR Primer (96% over 30bp)
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA103350.2252938629820929Illumina RNA PCR Primer (96% over 28bp)
TCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGC102470.22337554078156804No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT100370.2187977264394065No Hit
CTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGG92270.201140442548212No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC92200.2009878487368066No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC89760.1956688644535332No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA87070.18980490227238345No Hit
ATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGA84540.18428972594587453Illumina RNA PCR Primer (96% over 26bp)
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA77940.16990230944193827No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG67380.14688244303564024No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG62460.1361572780054332No Hit
CGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTA60600.13210264244523298Illumina RNA PCR Primer (95% over 23bp)
GGGGGCTGTGGCTTCTCTATAAGGACATGCCCCAACGTGACAACAGCTTG60280.13140507073595123No Hit
CGCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTACGA57420.12517052358424552No Hit
GGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGT55970.12200965177656255No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC55790.12161726769009157No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG55510.12100689244447002No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA54100.11793321710044727No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG50370.10980214686413178No Hit
CTCTGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCA49320.107513239693051No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG48920.1066412750564488No Hit
CGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC48230.10513713605831002No Hit
CCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGA47980.10459215816043366No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC46690.10178007220739155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACA979500.0144.51219145
CGTGCTA401800.0143.86394145
AGTGCTC645450.0143.03502145
CGTTGTA290900.0142.33397145
GGTGCTC626350.0142.19954145
TTCCAAT431500.0141.829575
CTTCCAA431350.0141.51074
TCCAATC438000.0140.969216
AGAGCGA197100.0140.131538
CCAATCC441750.0138.701267
CATCCTA216750.0138.54507145
CAATCCA438350.0138.371378
AATCCAG439350.0138.137429
TAACTTC287300.0138.074251
GCTAAGT68300.0137.873632
GCCTGGA429300.0137.75574145
AAACTTC158500.0137.624311
GAGCGAT194950.0137.510679
TAAGTGC68350.0137.353034
GAGAGCG202050.0136.734347
Produced by FastQC (version 0.11.5)