FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6333647
Sequences flagged as poor quality0
Sequence length114-124
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG1918253.0286657908153076No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC1817962.870321001470401No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG1452402.293149586644156No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC1031331.6283351440331297No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG939701.4836633617250852No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC706601.115628957534261No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG541930.8556365708414125No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC470280.7425105946068671No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT425930.6724877467910668No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATG404790.6391104524770642No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG379240.5987703451108026No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG353890.5587460115791107No Hit
GACAGACCCAGAAGACCCCTCCATCCTGTAGCACCTGCCATGAGCATCGG314560.4966490870110064No Hit
GCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCG308010.48630749392885336No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA305270.4819813923952503No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT291960.460966643704646No Hit
GAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCC289400.4569247386221556No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG258870.408721862775112No Hit
ACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGC254320.4015380080386545No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC250310.39520674265553474No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG249870.3945120402194817No Hit
GACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCCTCCT232220.36664499931871797No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA208670.3294626302981521No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT207210.32715748130579425No Hit
GAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG203970.32204194518576734No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC201760.3185526443137737No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA199600.31514228690042245No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG194590.30723215234445495No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC190880.30137454771318956No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT174760.2759231766468829No Hit
GGAGAGTCCTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCCTCC167130.26387640485805414No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC166430.2627711964370607No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA162340.2563136215201131No Hit
ATGAATACATGTCCTGGTATCGACAAGACCCAGGCATGGGGCTGAGGCTG159910.25247696943009296No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT154860.24450368010721152No Hit
GGCACAGACCCAGAAGACCCCTCCATCCTGTAGCACCTGCCATGAGCATC150650.23785664088952227No Hit
GGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAAT143930.227246640047985No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA143200.22609406555180608No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT130050.20533193592885743No Hit
AATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCT128810.20337413815452615No Hit
GCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGG126270.1993638104554927No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT125100.1975165335232608No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT122460.19334831890694257No Hit
GACACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC119810.18916431559889588No Hit
GGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT118840.18763281250123348No Hit
GAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAACAA118280.18674864576443873No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC116330.1836698508773855No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT114830.18130154711811378No Hit
AGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTC108130.17072312366003345No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC107600.16988632299842413No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA106000.16736013232186764No Hit
ACATCAACCCTGGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGT101820.1607604591793638No Hit
GAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATA98250.1551238962322971No Hit
CATGGGGCTGAGGCTGATTCATTACTCAGTTGGTGCTGGTATCACTGACC95390.1506083303979524No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC95130.15019782441301197No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT94950.14991362796189936No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT94760.14961364281905828No Hit
ACAAGACCCAGGCATGGGGCTGAGGCTGATTCATTACTCAGTTGGTGCTG94610.1493768124431311No Hit
TGAGGCTGATTCATTACTCAGTTGGTGCTGGTATCACTGACCAAGGAGAA94100.14857158916497873No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG88050.1390194306692495No Hit
ACTGCAGTGTGCCCAGGATATGAACCATGAATACATGTCCTGGTATCGAC87470.13810368654899777No Hit
AGGCTGCTGTCGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAG81230.12825154291042745No Hit
ATAACTCCATGTACTGGTATCGACAAGACCCAGGCATGGGACTGAGGCTG80270.12673582850449355No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC80220.12665688504585115No Hit
GAGAGGTAAGCAGCTGCCCCACTCTGAGAGGGGCTGTGCTGAGAGGCGCT80200.1266253076623942No Hit
GCTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCCTCCATCCTGT79900.12615164691053984No Hit
GAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCCTCCTGT79850.12607270345189747No Hit
CAGTGGTATCAACGCAGAGTACGAGGACCTGAAAAACGTGTACCAATCTG79840.12605691476016898No Hit
GAGACATCTGTGTACTTCTGTGCCAGCAGTACCGGGCCACGTAGCGGGGC78700.12425700390312248No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT77690.1226623460385462No Hit
CACTGCAGTGTGCCCAGGATATGAACCATGAATACATGTCCTGGTATCGA77610.12253603650471837No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA76740.12116242032434077No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC75350.11896779217408233No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC74260.11724682477567822No Hit
AGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCAGGTCCTGA72260.1140890864299826No Hit
GTCAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG72160.11393119951269783No Hit
GAGAGTCCTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCCTCCA71900.11352069352775739No Hit
CCAGGATATGAACCATGAATACATGTCCTGGTATCGACAAGACCCAGGCA71890.1135049048360289No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT70860.11187866958799567No Hit
GGAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCA69640.10995244919712135No Hit
TGTCACTCAGACCCCAAAATTCCAGGTCCTGAAGACAGGACAGAGCATGA69510.10974719620465113No Hit
ATGACACTGCAGTGTGCCCAGGATATGAACCATGAATACATGTCCTGGTA68950.10886302946785636No Hit
GCCTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCCTCCATCCTG68820.10865777647538614No Hit
GACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGTTGG68650.10838936871600201No Hit
GCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGC67640.10679471085142574No Hit
AGTGTGCCCAGGATATGAACCATGAATACATGTCCTGGTATCGACAAGAC67270.10621052925747204No Hit
CGACATCAACCCTGGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTG64900.10246860931782274No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG64710.10216862417498165No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT64490.10182127295695513No Hit
ACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGG64190.10134761220510079No Hit
GGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGT63910.10090552883670341No Hit
AGCAATCCAATCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGT63750.10065290976904775No Hit
GATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGA63630.10046344546830602No Hit
GAGAATGACCCCCTTGAGAGTCCTGTTCCCCTATCACCGATGCACAGACC63540.1003213472427497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG7800.0105.04131118
TATCCGC401.72804E-10103.130787
GAGATAC38600.099.694061
GAGAACT47250.098.405331
GGAGAGT120950.098.0801851
GTCTTTC11500.095.826841
GACCGAT7150.095.6082461
AGTGGTA595000.094.217212
GACAGAC335300.092.465171
GATTCCT24200.092.292671
GACATTA6100.091.777711
GGCACAG175600.090.611361
CAGTGGT629800.090.21171
GAATGCA67800.089.26561
AGTCATT26300.089.179264
GGCTTTC26050.089.1316451
GGTCCGA208.8135374E-488.3964161
GTTCCTT6100.087.913381
GAGGAGG136800.087.577931
TGCTTAC7950.087.4684456
Produced by FastQC (version 0.11.5)