FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6333647
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA93287314.72884421882053No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA6111309.648943176024808No Hit
TGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGGCTG1900443.000546130846888No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC1633972.579824862358133No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1060361.6741697161209015No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC492110.7769773086501347No Hit
TGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG471340.7441841959300858No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG414090.6537939357845488No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC353230.5577039579250311No Hit
CCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGAGCCCTG327210.5166217820475313No Hit
ATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT301850.4765816598241108No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT301200.4755553948617598No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT282210.44557266926937983No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA237440.3748866964009835No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT236120.37280258909282443No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC231300.365192439679698No Hit
TCCTGAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGA229550.36242941862721434No Hit
AACCACAGAGGATTTCCCGCTCAGGCTGCTGGCGGCTGCTCCCTCCCAGA188220.2971747557134144No Hit
CACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA184240.2908908564054801No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA182500.28814362404472493No Hit
AACCACAGAGGAGTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA181260.28618582627039363No Hit
TGATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACA179270.2830438766164265No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC168690.2663394407676967No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT162760.2569767465727092No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC155530.24556152245301957No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG143460.22650457153674652No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC136120.2149156718080436No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA135870.21452095451483166No Hit
ATTCTCAGGGCGCCAGTGCTCTAACTCTCGCTCTGAGATGAATGTGAGCA134840.21289471926679843No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA133960.21150531439469233No Hit
GAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCCAAAAGAACCCGA126590.19986904859080398No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC124770.19699550669622098No Hit
AAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC123060.19429564041065123No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC119090.18802752979444545No Hit
TGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGT117810.18600657725320024No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT115740.18273831806540528No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT115300.18204361562935226No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG114820.1812857584263853No Hit
ACCCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCTCCCAGA105920.1672338227880398No Hit
TTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA105860.16713909063766894No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC105140.16600230483321854No Hit
CCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAG100770.1591026465478736No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC95130.15019782441301197No Hit
AGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG91740.14484545791705788No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC91510.1444823180073029No Hit
ATTCTCAGGGCGCCAGGTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA88720.1400772730150575No Hit
AACCACAGAGGATTTCCCGCGCAGGCTGCTGTCGGCTGCTCCCTCCCAGA87900.13878260029332232No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA86190.13608273400775256No Hit
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG84760.1338249510905802No Hit
TTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTA84180.13290920697032846Illumina RNA PCR Primer (96% over 30bp)
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC83390.1316619003237787No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT82900.13088825442908328No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG82120.12965673647426199No Hit
ATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGC82040.12953042694043418Illumina RNA PCR Primer (96% over 29bp)
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC81130.12809365599314265No Hit
AACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCGCCCTCCCAGA79430.12540957839930136No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCGCTGAGATGAATGTGAGCA79040.12479381942189073No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA79010.12474645334670531No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC77800.12283602164755945No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC75510.11922041124173798No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC75260.11882569394852602No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG75210.11874675048988363No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCTCTCTGAGATGAATGTGAGCA74180.11712051524185038No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA74070.11694683963283713No Hit
ATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAG71770.11331544053528717No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA71400.11273125894133348No Hit
ATGCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA70880.11191024697145263No Hit
AACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCGGCTCCCTCCCAGA69660.10998402658057829No Hit
GTCTCCAGATTAAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG67960.10729994898673702No Hit
AACCACAGAGGATTTCCCGCTCAGGCGGCTGTCGGCTGCTCCCTCCCAGA66870.10557898158833291No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC64050.10112657052090208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)