FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848269
Sequences flagged as poor quality0
Sequence length113-123
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT304243.5865981192286878No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT273003.218318717293689No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT158391.8672142916928474No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG157941.8619093707302754No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC85871.012296806791242No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC71690.8451328529039727No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG70780.834405123846327No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT66390.7826526726781244No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC64900.7650874899353861No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT61740.7278351560648804No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG61530.7253595262823468No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG61120.7205261538497811No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT60400.7120382803096659No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC53150.6265701092460058No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC47690.5622037349001319No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC47350.5581955723950774No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG46240.5451101006873998No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG45920.5413377124473486No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT42780.5043211528418462No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT38530.454219121528666No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC37770.4452596994585444No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG37710.4445523766635348No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA35990.42427578987325953No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG35590.41956030457319554No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC35270.4157879163331444No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT32690.38537303614773144No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC31690.37358432289757143No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC30540.3600273026598874No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG29640.34941746073474333No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT28790.3393970544721073No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC27050.31888469341682885No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT26500.31240090112924085No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA25350.29884388089155683No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC24860.2930674113989784No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC24830.2927137500014736No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC23720.27962827829379594No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG23450.27644532571625274No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT23440.27632743858375114No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT22580.26618914518861353No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG22450.26465661246609273No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA22420.2643029510685879No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG21850.25758338451599666No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT21350.2516890278909167No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT20960.24709142972335427No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT20730.24438002567581746No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT20490.24155073449577905No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG20390.24037186317076306No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC19970.23542060360569583No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG19750.23282708669066063No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT19710.2323555381606542No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC19530.23023356977562542No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG19230.22669695580057742No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT19110.2252823102105582No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG19080.22492864881305338No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG18810.2217456962355102No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT18780.22139203483800537No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC18740.22092048630799901No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT18630.2196237278504814No Hit
GACAGTGACACTGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCAT18470.2177375337304558No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC18040.21266838703288696No Hit
GGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGTCTTGATTATCTCC17500.20630248187780056No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG17330.20429840062527335No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA16980.20017235098771735No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT16840.19852193113269492No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC16500.19451376862764053No Hit
GGACTCTGCTCTCTGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGT16470.19416010723013571No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC15940.18791208920755093No Hit
GATTCCAGCCTCTGACAAACTCTGCACATTAGGCCAGGAGAAGCCCCCGA15850.1868511050150365No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG15780.1860258950875253No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG15430.1818998454499693No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC15230.17954210279993726No Hit
GACAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGT15230.17954210279993726No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC15110.17812745720991807No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC14980.17659492448739728No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC14740.17376563330735886No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT14310.16869648660979006No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC14150.16681029248976445No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG13650.16091593586468445No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC13180.15537524063710922No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG13180.15537524063710922No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT12810.15101341673455002No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG12700.1497166582770324No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA12670.1493629968795276No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT12590.1484198998195148No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC12500.1473589156270004No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT12420.1464158185669876No Hit
GAGAGAAGGAACTGTGTCACCACAGAAACTTCTGCCTTCACCCATCCCTT12180.1435865273869492No Hit
GGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC12120.1428792045919396No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG11850.13969625201439637No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC11750.13851738068938038No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC11340.13368400825681476No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC11320.13344823399181158No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC11260.13274091119680195No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG11260.13274091119680195No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCGTTTGT10940.12896852295675074No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC10860.12802542589673793No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC10770.12696444170422355No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG10640.12543190898170273No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT10550.12437092478918835No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG10490.12366360199417874No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA10220.12048064941663553No Hit
GAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCC10090.11894811669411472No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT9810.11564727698406992No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC9680.1141147442615491No Hit
GGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTC9500.11199277587652029No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT9390.11069601741900269No Hit
ATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCTG9370.11046024315399951No Hit
GGCTCCTGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTG9290.1095171460939867No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC9120.10751306484145949No Hit
AGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGGCTCCTCTGCT9100.10727729057645628No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG9090.10715940344395468No Hit
GAGAATGACTTCCTTGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATA8980.10586264498643708No Hit
GCTTCCCCAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCC8860.10444799939641788No Hit
GTCTTCCCCAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCC8830.10409433799891307No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC8760.10326912807140187No Hit
GGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGC8730.10291546667389707No Hit
GGCTCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCA8670.10220814387888748No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT8650.10197236961388427No Hit
GAGAGCCCAGCACCTCGCCCAAAGGACCCCAGTCAGAGGCCCCATCTCAG8630.10173659534888108No Hit
GAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGT8580.10114715968637307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAA152.9047395E-4116.8502661
GAAGCTA900.0116.850261
ATCGTGC152.905421E-4116.843382
GGTTCAC1000.0105.165241
GGACGTG401.8371793E-10102.243981
GACTGGC401.8371793E-10102.243981
GAGGTAG1000.099.322721
GCTTCAA9250.099.164821
GAGAGCT11800.099.025641
GTTATCA600.097.375225
GACTGCA900.097.375211
GAGAACT8650.096.587211
ATAAACG1050.094.60988
AGTGGTA73450.094.015572
ATTGAGG1250.093.496748
GTATAAC501.0913936E-1193.4912346
GGATCTG10750.092.936721
GCATGCT1650.092.063841
GCTATTT1400.091.810911
GATTCCT12150.091.36441
Produced by FastQC (version 0.11.5)