FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848269
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT119451.4081617977316159No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC116251.3704379153311037No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC116111.3687874954760813No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA105621.2451238934819027No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC89621.056504481479342No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC84250.9931990913259827No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT83370.9828250236658419No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC79770.9403856559652658No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT64580.7613151016953349No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC64460.7599004561053156No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG63050.74327837042259No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG61680.7271278332698707No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG58170.6857494497618091No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG52540.619378994163408No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA52370.6173749129108809No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG52170.6150171702608488No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG51730.6098301364307784No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA51630.6086512651057624No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA51540.607590280913248No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT51220.6038178926731969No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT48320.5696306242477327No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA47860.5642078161526591No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC47120.5554841683475407No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC46520.5484109403974446No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT45680.5385084212673102No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA45520.5366222271472846No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA44660.5264839337521471No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT41420.4882885028216285No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG40650.47921119361900527No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA40420.4764997895714685No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG39370.46412164065880046No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA37400.44089787555598514No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA37390.4407799884234836No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA37230.438893794303458No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA35880.42297903141574195No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG32950.38843810159277303No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC31280.36875095046500583No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC30850.363681803767437No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG30770.3627387067074242No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC30360.35790533427485854No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG29960.35318984897479455No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA28600.3371571989545769No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC27190.3205351132718513No Hit
ATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG26510.3125187882617424No Hit
ACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA26470.31204723973173604No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC26120.30792119009418006No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG25940.3057992217091512No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC25670.302616269131608No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG25520.30084796214408405No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA25380.29919754228906165No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT25150.2964861382415248No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC25040.29518937978400717No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT24670.290827555881448No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC23810.2806892624863104No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT23530.2773884227762655No Hit
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG23290.27455913159622714No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT23090.2722013889461951No Hit
TAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAAC23050.27172984041618875No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG22690.2674859036461311No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC22610.26654280658611834No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC21980.2591159172385175No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC21970.2589980301060159No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA21870.2578191587809999No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT21110.24885973671087824No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA21080.24850607531337346No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA20350.23990031464075665No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA20200.23813200765323267No Hit
CTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCC19960.23530271647319423No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC19620.23129455396813983No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG19020.22422132601804382No Hit
TGAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCC18890.222688793295523No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG18860.22233513189801818No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG18650.21985950211548458No Hit
CGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCC18570.2189164050554718No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA18080.21313993556289337No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC17990.21207895137037897No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT17900.21101796717786456No Hit
TCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA17530.20665614327530535No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG17060.20111544804773013No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG16850.19863981826519655No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT16620.19592841421765972No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG16590.1955747528201549No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT16500.19451376862764053No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC16440.19380644583263093No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA16240.19144870318259893No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC16230.19133081605009733No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG16000.1886194120025605No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC15400.18154618405246448No Hit
TGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG15350.18095674838995648No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG15260.17989576419744208No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG15240.1796599899324389No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT14730.17364774617485726No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG14590.17199732631983486No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC14230.16775338954977725No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA13830.16303790424971326No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC13810.16280212998471003No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT13740.16197692005719883No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA13740.16197692005719883No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC13650.16091593586468445No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC13610.16044438733467803No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT13600.16032650020217643No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG13430.15832241894964924No Hit
TGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC13380.15773298328714122No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC13070.1540784821795916No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC12970.1528996108545756No Hit
ACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGC12910.15219228805956603No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA12660.149245109747026No Hit
ATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAG12580.1483020126870132No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC12470.1470052542294956No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG12360.145708495771978No Hit
ATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT12210.143940188784454No Hit
TCGATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA12200.14382230165195237No Hit
AGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT11900.14028568767690436No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA11670.13757428362936758No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC11660.13745639649686597No Hit
GAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGG11530.13592386377434515No Hit
AGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT11310.13333034685930995No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC11270.13285879832930356No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC11170.13167992700428757No Hit
CTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCT11020.12991162001676354No Hit
CAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGAGCC10950.12908641008925237No Hit
TGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCA10930.12885063582424916No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA10840.12778965163173475No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTG10730.12649289317421714No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA10710.12625711890921393No Hit
CGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTG10520.12401726339168354No Hit
AGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGC10510.12389937625918195No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA10450.12319205346417234No Hit
TGATGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCC10420.12283839206666752No Hit
GATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCA10400.12260261780166434No Hit
GTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGT10390.12248473066916273No Hit
TGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGT10320.12165952074165151No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG10310.12154163360914992No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG10250.12083431081414034No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG10090.11894811669411472No Hit
CTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTG9940.11717980970659071No Hit
CTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCATCC9900.11670826117658431No Hit
TAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCACCA9800.1155293898515683No Hit
TAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC9570.1128179858040315No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC9460.1115212273465139No Hit
AGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA8970.10574475785393549No Hit
TGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG8960.1056268707214339No Hit
GGATCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGA8900.10491954792642429No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG8750.10315124093890028No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA8730.10291546667389707No Hit
CCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATCCTG8700.10256180527639229No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG8600.10138293395137628No Hit
TCCTGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGA8590.10126504681887469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA180000.0144.72025145
CGTACTG55550.0143.78754145
GGTCACA228250.0143.69167145
AGTTGTA48350.0142.11359145
GGTGCTC71850.0141.93552145
AGTGCTC88150.0139.69948145
CGTTGTA31450.0138.73932145
CATCCTA38950.0137.89055145
TGTTGTA7950.0135.84476145
TTGACCA12900.0133.16263145
CGTGCTG10550.0132.59544145
GGGTTGC10100.0129.219649
CTTTCCA7150.0125.70119145
AGTGCTG18750.0124.86048145
TGTCTTG19000.0123.217575145
CGCTAAG1550.0116.939191
ACCCCAA4850.0116.608518
CCGATTC1900.0114.477321
AGGGTTG11800.0114.290028
GGTGACC4900.0113.938914
Produced by FastQC (version 0.11.5)