FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8988537
Sequences flagged as poor quality0
Sequence length112-123
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT3102353.4514515543519484No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1842172.049465891946598No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC1713331.9061277714048461No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG1646531.8318108942534252No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG1567101.7434427871855007No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG1472351.6380307496091966No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG1439971.602007089696577No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG1389351.5456909172204554No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA1331891.4817650525330206No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG1309401.456744295539975No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA1108151.2328480151998038No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC1065321.185198436631011No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC864400.961669290564193No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT837570.9318201616125071No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT779530.8672490306264524No Hit
GACAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGT648070.7209960864598989No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT632720.7039187801084871No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT616580.6859625765572306No Hit
GATTCCAGCCTCTGACAAACTCTGCACATTAGGCCAGGAGAAGCCCCCGA604510.6725343623773257No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT521060.5796938923431032No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT462290.5143106158432679No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA443060.4929167004597077No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC429160.47745255985484625No Hit
GGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGTCTTGATTATCTCC426520.4745154856680236No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG422420.46995412045364No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT415900.46270043723466897No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA403260.44863808203715466No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC395920.4404721257753069No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT382530.4255753745019907No Hit
GAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCC378750.42137001827994924No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT360860.4014668905518218No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT344770.3835663134056187No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC342440.38097412293012756No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA336860.37476621612616157No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT336760.37465496331605463No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG328170.36509834692787047No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC320850.35695464122804416No Hit
GGACTCTGCTCTCTGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGT308690.34342629951904297No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC293460.32648249653975947No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT281570.313254537418047No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC275190.3061566081332257No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC271130.30163974404288485No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT266610.2966111170260522No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT262120.2916158658522516No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC255670.2844400596003554No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG246380.2741046735414228No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG244140.27161261059502784No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC243390.270778214519226No Hit
GGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGC242480.269765813947253No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG227310.2528887626540337No Hit
AGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGAC214660.23881528217550865No Hit
GACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGA212460.23636772035315645No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT211920.23576695517857912No Hit
CAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG211790.2356223265254401No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC211090.2348435568546917No Hit
GGATTATCTCCAGGAACCTCCCACCTCTTCATTCCAGCCTCTGACAAACT204340.2273339921724748No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG198740.2211038348064874No Hit
ACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCTT198200.22050306963191008No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG196200.2182780134297717No Hit
GGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTC192290.21392802855459123No Hit
GAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCTTCCAA185010.20582882397880767No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT181050.20142321269857374No Hit
GAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAG179170.1993316598685637No Hit
GAGGAACCTCCCACCTCTTCATTCCAGCCTCTGACAAACTCTGCACATTA177000.1969174738892436No Hit
GGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGAC170880.19010880191070026No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC170630.18983066988543296No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC170010.18914090246277007No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT164940.18350038499034937No Hit
GGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCC164510.18302199790688964No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC163020.18136433103629657No Hit
ACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG156580.17419965006541108No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG156550.17416627422237901No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC154290.1716519607139627No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA152910.17011667193448723No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG152510.16967166069405953No Hit
GAGAGGTAAGCAGCTGCCCCACTCTGAGAGGGGCTGTGCTGAGAGGCGCT148780.16552193087707154No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT147820.16445390390004513No Hit
GCACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGA146980.16351938029514704No Hit
GGCAAAAGCCCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGA146590.16308549433573005No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT143860.1600482926198112No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA143250.15936965047815901No Hit
GCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAG140590.15641032572931501No Hit
GGAGCTGAGTCTGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCTGGG139530.15523104594218168No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT138450.154029515593027No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC137040.15246085097051945No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA136380.1517265824238138No Hit
GGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGG132260.1471429666474088No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG127750.14212546491158684No Hit
GAAGCCTGTCTTGATTATCTCCAGGAACCTCCCACCTCTTCATTCCAGCC124700.13873225420332586No Hit
GAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCC123630.13754184913518183No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT123610.13751959857316046No Hit
GAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCA121690.13538354461910765No Hit
GGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGGGT120780.13437114404713468No Hit
GGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCT117870.1311336872730234No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC116420.1295205215264731No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG116140.12920901365817372No Hit
GGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGGGTGCTGG115240.1282077383672115No Hit
GACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGG113020.12573792598283792No Hit
GACACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCT112830.12552654564363477No Hit
GGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGA112640.12531516530443165No Hit
GACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCT111720.12429163945144799No Hit
GCTGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGTCTTGATTATCT110840.12331261472250712No Hit
GACCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGACTCTG109700.12204433268728826No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT106030.11796135455636442No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA103770.11544704104794808No Hit
GCTCTTCATTCCAGCCTCTGACAAACTCTGCACATTAGGCCAGGAGAAGC103170.11477952418730657No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC101900.11336661349894872No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC100780.11212058202575124No Hit
ACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGG100460.11176457303340911No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG97980.10900550334275755No Hit
GACTCTGCTCTCTGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGTC97790.10879412300355443No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT96470.1073255859101431No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC94960.10564566847752865No Hit
GAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGA94130.10472227015364124No Hit
GGAGTCTGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCTGGGCCTCC93790.10434401059927774No Hit
CGTCTGGGCGGAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCT93270.10376549598672175No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC91620.10192982461995762No Hit
GGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGT91120.10137356056942304No Hit
GGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATG89990.10011640381521486No Hit
GACACCAGCTCCTCTGGGGTTTGCCACACTCATGATGCACTGTGTAGCAA89980.10010527853420417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)