FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8988537
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA7146177.950314940017492No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA4489414.994594782220956No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT3828014.258768696173805No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT3171193.52803798882955No Hit
TGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACAC2439852.7144016873936216No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA2178042.4231307052527016No Hit
ATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT2104592.3414155162291705No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA1499331.6680467577760432No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1036491.153124251477187No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC1003591.1165220769520112No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG715930.796492243398453No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC700710.7795595657001801No Hit
TAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGC692920.7708929717928512No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA661790.7362599720065679No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG655640.7294179241849925No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG444320.49431848586705496No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG407380.45322169781355964No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC352860.3925666657432683No Hit
AGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT330410.3675904098742654No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC323440.3598360890098133No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG323140.3595023305794925No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT316690.35232652432759637No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA314440.34982333610019073No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA305510.339888460157643No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC282700.31451169417225516No Hit
GGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACAC257490.28646486074430133No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA221540.24646947551086457No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC203500.22639946856757667No Hit
TTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAG202080.22481967866405847No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA194880.21680947633636044No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA190560.2120033549397416No Hit
CGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTG186550.2075421172544542No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC170730.1899419226955399No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA167170.18598132265573364Illumina RNA PCR Primer (96% over 28bp)
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC164550.1830664990309324No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC158210.17601307087015383No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC156380.17397714444519727No Hit
ATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGA155220.17268661184795703Illumina RNA PCR Primer (96% over 26bp)
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG151730.1688038887752256No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT149650.16648983032500173No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC147790.16442052805701307No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC139150.1548082852637754No Hit
ATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGCCAG133310.14831112115353143No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG133280.14827774531049936No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC133190.14817761778140312No Hit
AAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGC132960.14792173631815722No Hit
AGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG131470.14626406944756415No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG124490.13849862330210133No Hit
CTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGG122650.13645157159613405No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG121100.13472715303947683No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA119620.13308061144989444No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA118150.13144519514132277No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC110980.12346836865665681No Hit
GGCTGGGAGAGCGAGGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT110530.12296773101117568No Hit
TCCCTGTGAGGGAAGAGTTCCCATGAACTCCCAACCTCTGCCTGAATCCC109090.12136569054563608No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT106420.11839524051578137No Hit
AGGCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA106390.1183618646727493No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC105270.11711583319955184No Hit
GGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG104350.1160923073465682No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAAC104250.11598105453646126No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGGCGCCCA101960.11343336518501287No Hit
ATTCTCAGGGCGCCAGTGCTCTAACTCTCGCTCTGAGATGAATGTGAGCA100030.11128618594994936No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA98120.10916125727690724No Hit
GTACAGCGTCTCTCTGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT96670.10754809153035695No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG91820.10215233024017144No Hit
CAATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGC91640.10195207518197902No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC90640.10083954708090984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA578950.0144.38048145
GGTCACA843600.0143.89488145
TGTTGTA590500.0142.18248145
AGAGCGA645950.0142.157468
CGTTGTA216050.0141.78168145
AGTTGTA1098850.0141.77628145
GAGCGAT634950.0141.640679
GAGAGCG651600.0141.473197
AGTGCTC3228400.0140.99937145
CATCCTA389850.0137.58752145
TGGGAGA619300.0137.487464
CTCAACA320500.0137.430799
GCTGGGA620750.0137.174162
GGGTTGC102450.0136.870629
GGTGCTC288750.0136.38374145
GCTCAAC323800.0135.581568
GGTCGAT63600.0135.321442
GGAGAGC676150.0134.935496
TTGCAGA168550.0134.863346
TGTCTTG918250.0134.63599145
Produced by FastQC (version 0.11.5)