FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836339
Sequences flagged as poor quality0
Sequence length114-124
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC529516.331284323701274No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG435975.212838334694425No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT165871.9832866815968166No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC138921.6610489287238788No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG133681.5983949092413483No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG129701.550806550932098No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC103871.2419604968798537No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT101341.2117096057938228No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC91631.0956083597679889No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT88541.0586616192716112No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG83651.0001925056705476No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG79120.9460278666904209No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC71740.8577861369612083No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG68130.8146218220123658No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC66550.7957299611760303No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA62160.7432392845484904No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG55040.6581063420455103No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT54680.6538018674245731No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT52150.6235509763385421No Hit
GAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCT50430.60298515314962No Hit
AGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT43190.5164173857729939No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA43140.5158195420756416No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC42150.5039822368680643No Hit
GGCTTCTGCTGCTTCTGGGGCCAGGCTCCGGGCTTGGTGCTGTCGTCTCT41090.4913079504841936No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC39740.4751661706556791No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG38080.45531775990357976No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC31230.3734131733663024No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC30630.36623904899807375No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG30340.3627715555534299No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT30270.36193457437713655No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC29540.35320605639579167No Hit
GGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT28750.3437601259776239No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT28000.33479247051733807No Hit
TGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCTGGGGCCAG26770.3200855155624693No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT25710.30741122917859864No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC25190.3011936547261338No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT25130.3004762422893109No Hit
GGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATG25000.2989218486761947No Hit
GACACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCT24050.2875628184264993No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG23960.286486699771265No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA23960.286486699771265No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC23760.28409532498185547No Hit
ACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGG23050.27560594447945147No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC22420.2680731138928114No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA21210.2536052964168836No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA21040.25157262784588547No Hit
GGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC20830.24906168431700543No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT20620.2465507407881254No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT19640.23483300432001858No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG19010.22730017373337846No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC18990.2270610362544375No Hit
GAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTG17680.2113975313838049No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT17430.20840831289704295No Hit
GATCACGGAAGATGCTGCTGCTTCTGCTGCTTCTGGGGCCAGGCTCCGGG17380.20781046919969054No Hit
GATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAG17150.20506038819186959No Hit
GCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT16990.2031472883603419No Hit
ATTCTGTGATCAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCAC16910.2021907384445781No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG16670.19932108869728662No Hit
GACACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC16420.19633187021052467No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG15910.19023386449753033No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT14820.17720087189524822No Hit
GCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAA14530.17373337845060435No Hit
GGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGT14180.16954847256913763No Hit
TCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTG14020.16763537273760998No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT13780.1647657229903185No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT13450.16081995458779275No Hit
ACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCT13410.16034167962991083No Hit
GGATCAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCT12910.15436324265638693No Hit
GATCCTGCCCTGACTCTGTCATGGGCACCAGGCTCCTCTGCTGGGCAGCC12700.1518522991275069No Hit
GAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATGCACAGACC12580.15041747425386118No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC12360.14778696198551067No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC12330.14742825576709923No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC12320.14730868702762875No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT12260.14659127459080587No Hit
GAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCTGGGGCCAGGCT12030.1438411935829849No Hit
GAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGGC11750.1404932688778115No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG11750.1404932688778115No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC11610.13881930652522484No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT11510.13762361913052004No Hit
GAATGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCA11490.1373844816515791No Hit
GGTCACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTG11210.1340365569464057No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC11070.13236259459381902No Hit
GCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCT11070.13236259459381902No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT11060.13224302585434852No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC10990.1314060446780552No Hit
GCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGG10680.1276994137544704No Hit
CATAACGCTATGTATTGGTACAAGCAAAGTGCTAAGAAGCCACTGGAGCT10640.12722113879658845No Hit
GCAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCC10500.1255471764440018No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG10260.1226775266967103No Hit
TGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCC10230.12231882047829888No Hit
GCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCT10070.12040572064677121No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC10020.11980787694941884No Hit
GGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCTGGGGCCAGGCTCC9960.11909046451259596No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTG9780.11693822720212736No Hit
GGAATGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACC9680.11574253980742258No Hit
GGACTCTGTCATGGGCACCAGGCTCCTCTGCTGGGCAGCCCTGTGCCTCC9630.11514469611007021No Hit
GGCTCCTGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTG9480.11335116501801303No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT9460.11311202753907207No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT9400.11239461510224921No Hit
GCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC9380.11215547762330824No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG9370.11203590888383777No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT9100.10880755291813486No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG9010.10773143426290056No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT8990.1074922967839596No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC8940.10689445308660724No Hit
GGTCAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAG8930.10677488434713675No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG8810.10534005947349101No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG8740.10450307829719767No Hit
GGCTGCTGCTTCTGCTGCTTCTGGGGCCAGGCTCCGGGCTTGGTGCTGTC8730.1043835095577272No Hit
GAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTT8690.10390523459984527No Hit
GGCTGCTTCTGCTGCTTCTGGGGCCAGGCTCCGGGCTTGGTGCTGTCGTC8460.10115515359202429No Hit
TGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCTGGGGC8410.10055730989467189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTAG152.8143835E-4117.781688
ATTCGTA152.8157234E-4117.767594
GAAGCTA251.2844794E-7117.760561
GACTTCG251.2844794E-7117.760561
GACTGGC251.2844794E-7117.760561
GACGGAA650.0117.760551
GCACTAC205.9978465E-6117.7605441
TATTAAC700.0109.3687067
TTAACGC700.0109.3687069
GAGTCAG12850.0103.555971
GCTTCAA4450.0103.205881
GAGGAGC4400.0103.040481
AGTCATT1400.0100.943664
GAGAGCC3700.0100.25561
TCAGGAG1650.099.9180453
GAGAACT4950.099.9180451
GTCTTTC2600.099.643551
CCTCATA700.099.23009118
GACAACT7750.098.766911
CTAAACT303.8035614E-798.15148
Produced by FastQC (version 0.11.5)