FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836339
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA18107621.65102906835625No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC618687.397478773559525No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG293373.50778810984541No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC166501.9908195121834567No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT148401.7744000937418916No Hit
ATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAG119141.4245419620512736No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA101171.2096769372228247No Hit
CTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGC97551.1663930535345117No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG93261.1150980643016768No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC83370.9968445809653741No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC52700.6301272570094185No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA50820.6076483339889686No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA50230.6005937783602103No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG48050.5745277931556462No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC43190.5164173857729939No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG39590.47337263956362197No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG39360.470622558555801No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT38000.45436120998781593No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC34460.41203387621526677No Hit
TCCTGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGA30320.36253241807448894No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC30120.36014104328507934No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC29960.35822794345355174No Hit
GGTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA29850.3569126873193765No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA28010.3349120392568085No Hit
CTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCCT25650.30669381674177576No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC24210.289475918258027No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC23450.2803886940582706No Hit
CTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAGAAGACTCGGCCC22730.2717797448163962No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA21820.26089898952458274No Hit
CACATATGAGAGTGGATTTGTCATTGACAAGTTTCCCATCAGCCGCCCAA20920.2501378029722397No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT19890.23782222280678053No Hit
TTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA19400.2319633545727271No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC19160.22909370482543562No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA19000.22718060499390796No Hit
AATGTCTCCAGATTAAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG18180.21737596835732878No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG17920.21426718113109638No Hit
TAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGTCTG17580.2102018439891001No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG17560.20996270651015914No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTAC17450.2086474503759839No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC16690.1995602261762276No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT16230.19406006416058558No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT15950.1907121394554122No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC15880.18987515827911888No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA15550.1859293898765931No Hit
GCTGTGTGCCCAGGATATGAACCATGAATACATGTACTGGTATCGACAAG15420.1843749962634769No Hit
TGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA15330.1832988776082426No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC15320.18317930886877212No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC14200.16978761004807857No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC14160.1693093350901967No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA13970.1670375290402576No Hit
AGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTC13850.16560270416661188No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTAC13430.1605808171088518No Hit
GTGTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA12900.15424367391691646No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG12870.15388496769850504No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC12420.14850437442233352No Hit
GCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGGG12220.14611299963292396No Hit
TGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGC12170.14551515593557157No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC12000.14348248736457347No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC11490.1373844816515791No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG11450.13690620669369716No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG11310.1352322443411105No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA11190.13379741946746476No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC11180.13367785072799426No Hit
GTATCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA11120.1329604382911714No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC11020.13176475089646664No Hit
CTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGC10770.12877553240970468No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGGTGGAGTCGG10660.12746027627552942No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT10660.12746027627552942No Hit
CTCACCTGACTGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC10570.12638415762029512No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC10090.12064485812571218No Hit
GTTTCTCATCAACCCTGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA10060.12028615190730074No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA9840.11765563963895025No Hit
TGATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACA9710.11610124602583402No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG9590.11466642115218828No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT9430.11275332132066064No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC9270.11084022148913299No Hit
CACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGG9200.11000324031283965No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC9020.10785100300237105No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA8860.10593790317084341No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG8700.10402480333931576No Hit
CTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA8500.10163342854990619No Hit
GTTTCTCATCAACCATGCCAGCCTGACCTTGTCCACTCTGACAGTGACCA8460.10115515359202429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)