FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16151092
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA2639941.6345272505413257No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2388371.478766884616842No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG1671521.0349269263031873No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1665271.0310572189174578No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT1604330.9933260240236388No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA1603640.9928988083282542No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC1501270.9295160971159101No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC1498050.9275224238707823No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA1479030.9157461303545296No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG1469960.9101304109963586No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA1415220.8762379658291835No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT1257090.7783312732043133No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC940250.5821587791091772No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC888660.5502166664644099No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA855290.5295555247905219No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC823350.5097797721664887No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA817830.5063620466034123No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG815230.5047522483309488No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG813180.5034829843084294No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC799260.49486437201893224No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA718290.44473153889532674No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC716790.4438028091227516No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT678160.4198849217130334No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA677710.4196063027812609No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG592150.36663155655357543No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC558340.345697987479732No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA505320.3128704857851098No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT502520.3111368568763029No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT491510.30431998034560137No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG489660.30317454695942536No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG475850.29462404151991706No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT454240.281244141263018No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT453730.28092837314034247No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG427290.2645579630157515No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG425970.26374068081588536No Hit
GGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTAC414970.2569299958170011Illumina RNA PCR Primer (95% over 22bp)
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA408530.2529426493267452No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA400120.24773557106850733No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG391590.24245419442846342No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG388920.24080105543327965No Hit
TTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTA388250.24038622280152946Illumina RNA PCR Primer (96% over 30bp)
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA387560.2399590071061449No Hit
TACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAG382540.2368508581339268Illumina RNA PCR Primer (96% over 25bp)
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG377410.23367460231171985No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC373160.23104320128942363No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC372680.23074600776219958No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT372120.23039928198043824No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA370600.22945816914422876No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC370400.2293343385078854No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC352010.21794811149611432No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA344140.21307537595600345No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC340100.2105739971018678No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG337960.20924900929299392No Hit
TCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGA337710.20909422099756472No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC336420.20829551339315014No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA333690.2066052252070634No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT330870.20485921323462214No Hit
GAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTA321810.19924968540826837No Hit
ATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGC318190.1970083508904537Illumina RNA PCR Primer (96% over 29bp)
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG316410.1959062582269979No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG313420.19405499021366482No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG312930.1937516051546236No Hit
CTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGC304480.18851976076911703No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC304110.18829067409188185No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA298020.1845200312152268No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA297130.18396898488349891No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC295110.18271829545643106No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG294310.18222297291105766No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC290680.17997544686142586No Hit
TTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCA285330.17666297733924122Illumina RNA PCR Primer (96% over 30bp)
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG284460.17612431407114765No Hit
TGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGA282260.17476217707137076No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT279470.17303473969438105No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT275490.17057051003114837No Hit
TTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGT274420.16990801612671144Illumina RNA PCR Primer (96% over 30bp)
AGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGC272550.1687501996769011No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT268830.16644694984091477No Hit
GATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAG268690.16636026839547444Illumina RNA PCR Primer (96% over 27bp)
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA265310.1642675306412718No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA264230.16359884520501772No Hit
CGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTA262030.16223670820524086Illumina RNA PCR Primer (95% over 23bp)
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG259120.1604349724464451No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC258920.16031114181010175No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC258270.15990869224198587No Hit
ACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC252180.15613804936533082No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA247730.1533828177066913Illumina RNA PCR Primer (96% over 28bp)
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC246100.152373598020493No Hit
AATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACG244590.15143867671610067Illumina RNA PCR Primer (96% over 30bp)
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT244090.1511291001252423No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC241430.14948215266187573No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC241240.14936451355734956No Hit
AACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCTG237350.14695600768047137No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA236840.14664023955779584No Hit
ACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGT235170.14560625374432887Illumina RNA PCR Primer (95% over 24bp)
TTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAA234610.1452595279625675Illumina RNA PCR Primer (96% over 30bp)
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG234180.14499329209442927No Hit
TAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAAC230350.14262193540845414No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC230220.14254144549483094No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT226040.1399533851952549No Hit
TTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGG225770.1397862138361914Illumina RNA PCR Primer (96% over 30bp)
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG225570.13966238319984803No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC222450.13773062527289176No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA220560.1365604257594471No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC213270.13204679906473196No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG213050.1319105853647543No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT209910.1299664443741637No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC205900.1274836401154795No Hit
GCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC201350.12466649313866829No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA199750.12367584804792148No Hit
TAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAAC198790.12308146099347338Illumina RNA PCR Primer (96% over 30bp)
ATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGA198730.12304431180257037Illumina RNA PCR Primer (96% over 26bp)
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG198720.12303812027075323No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT193990.12010952572123297No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG193960.12009095112578147No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC193140.11958324551677373No Hit
GGGGGCTGTGGCTTCTCTATAAGGACATGCCCCAACGTGACAACAGCTTG188460.11668560862633934No Hit
CCAGAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTT187350.11599834859463373No Hit
TTTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTG187310.11597358246736506Illumina RNA PCR Primer (96% over 30bp)
TTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTAT185280.11471670150848005Illumina RNA PCR Primer (96% over 30bp)
ACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGC184160.11402324994495727No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG181410.11232057869523622No Hit
TCCTGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGA181080.11211625814526967No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT178470.11050026834098896No Hit
AGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA178300.1103950123000971No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA176400.10921862125483528No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG174250.10788744191414426No Hit
TTCAGCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTA173500.10742307702785668No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT170720.10570183118268411No Hit
CTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCC168380.10425301273746693No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA166520.10310138781947376No Hit
CGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCC163530.10125011980614065No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG163310.10111390610616298No Hit
TGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG162600.10067430734714408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA2563350.0144.02629145
GGTCACA2424500.0143.1905145
CGTTGTA900150.0142.1997145
CATCCTA1248500.0140.88762145
GGTGCTC1220400.0140.57446145
CGTACTG851850.0139.48323145
AGAGCGA276100.0138.23428
CTTTCCA186800.0137.65134145
AGTTGTA1429250.0137.22758145
TGTTGTA297650.0136.5937145
CGTGCTG257850.0136.34456145
AGTGCTC1712050.0133.52852145
TTGACCA208400.0133.15617145
GAGAGCG288850.0132.358387
GAGCGAT287800.0130.146069
GGGTTGC122700.0120.9596949
GCCTGGA1583800.0119.85568145
GCTGGGA304300.0116.801792
CGAGGAT124800.0116.2532961
AGGATCG134350.0115.544443
Produced by FastQC (version 0.11.5)