FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389457
Sequences flagged as poor quality0
Sequence length114-123
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC490713.5316674067639373No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT481363.464374932077783No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT293402.111616264483176No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC259781.8696512378576668No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG240211.7288048496642932No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT224771.6176823032306866No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG218411.5719090263318691No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG215871.553628503796807No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT215321.5496701229329155No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT200881.445744632615475No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG176281.2686970521577854No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA164531.1841316427928321No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA157461.1332484560515368No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG155231.117199020912486No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG149711.0774712711512484No Hit
GATTCCAGCCTCTGACAAACTCTGCACATTAGGCCAGGAGAAGCCCCCGA143941.0359442573609692No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC137140.9870042757710387No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT107490.7736115619267095No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT101250.7287019317618321No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC101240.7286299612006705No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT99730.7177624064652595No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC93800.6750838636963936No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA84150.6056322721753894No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT82430.5932533356555835No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT82410.5931093945332602No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC79640.5731735490914797No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG61520.4427628922665473No Hit
GACCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGACTCTG59110.4254179870265867No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT57150.4113117570389008No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT55010.39591005695030507No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT52830.3802204746170626No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT52100.3749666236522613No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC51420.37007262549326825No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT50300.36201192264316207No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA49670.3574777772899773No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC49330.3550307782104808No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT48670.3502807211738111No Hit
GACAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGT48530.3492731333175478No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT48060.34589051694294964No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT45000.32386752522748097No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG44480.3201250560470745No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC43660.31422347003181816No Hit
GACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTG43010.3095453835563101No Hit
GAGTCCTCTCACCACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGT42930.3089696190670168No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC41870.30134073958388063No Hit
GAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCC41700.30011724004413237No Hit
GAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGA39700.28572312781179987No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC37490.26981763379507245No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC36280.2611091958945113No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC35410.25484775707344665No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG35370.2545598748288No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC34990.25182499350465687No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG34510.24837040656889706No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG34080.2452756724389456No Hit
CAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG34030.24491581963313724No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG34010.24477187851081392No Hit
GGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGTCTTGATTATCTCC33990.24462793738849062No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC33810.24333246728758068No Hit
GACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCT33250.23930211586252761No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG33230.2391581747402043No Hit
GAGTCCTGACCTGCCTGACTCTGCCTGGAACACCAGGCTCCTCTGCCATG31430.22620347373110503No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT30870.22217312230605196No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG30380.21864656480913047No Hit
GAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCTTCCAA30380.21864656480913047No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC30010.215983654046149No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT28250.20331683528169636No Hit
GGCAAAAGCCCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGA27740.19964633666245157No Hit
GCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTCC27180.1956159852373985No Hit
GAGCAAAAGCCCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGG27040.19460839738113522No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA26880.1934568684025486No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT26800.19288110391325533No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC26610.19151366325118374No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA26600.1914416926900221No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT26450.19036213427259713No Hit
GGCTTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGT26050.18748331182613065No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG25930.1866196650921907No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC25640.18453251881850247No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA25440.18309310759526923No Hit
ACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCTT25440.18309310759526923No Hit
GGACTCTGCTCTCTGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGT24500.17632787484607296No Hit
GAGGAACCTCCCACCTCTTCATTCCAGCCTCTGACAAACTCTGCACATTA23410.16848308367945175No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT23340.16797928975132012No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC23110.16632396684460188No Hit
CTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTCCT22850.16445273225439866No Hit
GACACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCT22640.16294135047000374No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC22430.16142996868560883No Hit
GGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGACTCTGCCTGGAACACC21970.15811932287217237No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT21930.15783144062752572No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA21330.15351320695782597No Hit
GGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGC21190.1525056191015627No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG21070.15164197236762275No Hit
GAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGACTCTGCCT20910.15049044338903614No Hit
GAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAG20250.14574038635236644No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC20120.1448047690572648No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG19950.14358126951751654No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC19480.1401986531429184No Hit
GCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAG19340.13919106528665515No Hit
GGATTATCTCCAGGAACCTCCCACCTCTTCATTCCAGCCTCTGACAAACT18760.13501677273927873No Hit
GGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGAC18530.13336144983256049No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC18380.13228189141513555No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC18070.13005080401912403No Hit
GGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCC18020.1296909512133157No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC17650.1270280404503342No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC17630.12688409932801087No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG17590.12659621708336422No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC17570.1264522759610409No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC17450.12558862922710093No Hit
GCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGACTCTGCC17440.12551665866593928No Hit
GACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGG17440.12551665866593928No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT17260.12422118856502935No Hit
GAGAAGCTGTGCGGATGGCTGTCTGCCCGGAAGCCTGCCCCCTCTCGCTC16210.11666427964305481No Hit
GGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTC16030.11536880954214489No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG15820.11385742775774997No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT15690.11292181046264836No Hit
GAAGCCTGTCTTGATTATCTCCAGGAACCTCCCACCTCTTCATTCCAGCC15500.11155436980057676No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT15440.1111225464336068No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG15380.11069072306663683No Hit
GATCCTTCCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCC15100.10867554735411028No Hit
GCACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGA15050.10831569454830196No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC14540.10464519592905717No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC14470.10414140200092553No Hit
GAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCC14450.10399746087860222No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA14240.10248607909420729No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC14240.10248607909420729No Hit
GGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTG14150.10183834404375233No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC14140.10176637348259067No Hit
GAAAATTAGGAAGCCTGTCTTGATTATCTCCAGGAACCTCCCACCTCTTC14030.1009746973098124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACTA152.9116246E-4116.784761
ACGGGGT152.9116246E-4116.784762
GATTCCG152.9116246E-4116.784761
GGCGAAT356.366463E-11116.784751
GGCTTTC54900.0115.4020541
GACTGCA4350.0114.1000441
GACATTA57100.0111.4670641
GATTCCT12400.0111.133871
GAGAGTT9000.0110.945521
GAGAACT18650.0110.835931
GGCAAAA2900.0110.744171
GAAATAG850.0109.915071
GGATCTG12250.0108.204651
GGACGTG650.0107.8013151
GACTACG650.0107.8013151
GGTTCAC4450.0106.2872541
GACTTCG550.0106.167961
GCTTTCC3150.0105.662391
GGTAATA500.0105.106281
GAATGCG500.0105.106281
Produced by FastQC (version 0.11.5)