FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389457
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT595844.288293916256494No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA390632.81138603065802No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA335712.4161237087581693No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT253551.8248135782539512No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT222711.602856367631384No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA220221.58493569790213No Hit
TAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGC139461.0037014459605442No Hit
TGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACAC136530.9826140715401771No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC125920.9062533061476533No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG109810.7903087321162152No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG96020.6910613282742827No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC93530.6731406585450288No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC80710.5808743991357775No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA76720.5521581452322742No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT72380.5209229216881127No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA67220.4837861121286949No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT64930.46730485362267415No Hit
ATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGCCAG60390.43463021885527947No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA58400.4203080771841086No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC57210.4117435804058708No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG48990.35258377913098427No Hit
AGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT48870.3517201323970443No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC44680.32156446727030774No Hit
AAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGGAGC43330.3118484415134833No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA40510.29155274326589453No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC39620.28514736332250656No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC37940.27305630904734723No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG37490.26981763379507245No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA36170.260317519721733No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG35380.2546318453899617No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA34340.2471469070291488No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAAC30760.22138144613327365No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG30730.22116553444978868No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC27570.19842283712270334No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA27040.19460839738113522No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT26400.19000228146678882No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA24500.17632787484607296No Hit
CTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGG24280.1747445225005164No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC22190.15970267521772896No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA22120.1591988812895973No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG20050.14430097512913317No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC19920.14336535783403156No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC19760.14221382885544498No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT19760.14221382885544498No Hit
CGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTG19690.14171003492731332No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC19530.14055850594872674No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA19250.1385433302362002No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG19020.13688800732948195No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC18720.13472889049463208No Hit
CTTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT18190.13091445075306396No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC17800.12810759886775913No Hit
CGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT16640.1197590137730063No Hit
ATTCTCAGCTCAACAGTGCAGTGACTATCATTCTGAACTGAACATGAGCT16460.11846354367209637No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG16270.11709610301002477No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC16030.11536880954214489No Hit
CTTAAACCTTCACCTACACGCCCTGCAGCCAGAAGACTCAGCCCTGTATC15960.11486501561401324No Hit
TTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAG15260.10982707633269688No Hit
AGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG14970.10773993005900866No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG14820.10666037164158372No Hit
TCCCTGTGAGGGAAGAGTTCCCATGAACTCCCAACCTCTGCCTGAATCCC14150.10183834404375233No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC14000.10075878562632741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG100.0070954575144.97061
CGTGCTA83450.0144.27193145
GGTCACA157800.0143.85913145
AGTTGTA198100.0142.4181145
CGTTGTA52800.0141.208145
AGAGCGA48100.0141.198098
TGTTGTA66400.0140.79427145
TGTCTTG132900.0140.3063145
CATCCTA74100.0139.27936145
CTCAACA111050.0139.156319
GAGAGCG49250.0138.936287
GAGCGAT48500.0138.688529
GCTCAAC112100.0138.499488
GGTGCTC65350.0138.39519145
AGTGCTC408500.0137.47296145
GGGTTGC21100.0135.346829
AGGACAA12550.0135.151873
GGGTACA90000.0132.648092
GGTCGAT8050.0132.364462
CGTACTG22650.0132.24582145
Produced by FastQC (version 0.11.5)