FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795409
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC477631.7086229600033482No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT425481.5220670749790102No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA412591.4759557545961968No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT394691.411922190992445No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA356621.2757346062776502No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC353281.2637864441303581No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA338761.2118441344361415No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG289961.0372721844996564No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC261120.9341030239224385No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA255860.9152864571874814No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC202470.7242947275336096No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA191470.684944492916779No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG188620.6747492048569637No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG175330.6272069668517201No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC165660.5926145333294699No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA157610.563817316178062No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG155800.5573424139365654No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC155270.5554464480868452No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA150440.5381681177960005No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC145990.5222491592464644No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA144450.5167401264001081No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT127940.4576790015343014No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG126650.453064292201964No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG125140.44766257817728994No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC122140.4369306960090634No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG120600.4314216631627071No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG118410.4235873891799018No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG113220.40502123302886983No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT112990.4041984553959725No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG112980.4041626824554117No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG104620.3742565041466204No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT103820.37139466890176No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC101250.3622010231776459No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT100990.36127092672306627No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC100170.35833754559708436No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC95800.34270477057203436No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA94950.3396640706243702No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC94100.336623370676706No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC92750.331794023701004No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT92240.3299696037324055No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG92050.32928991786175116No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG91640.3278232272987602No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT87270.31219045227371023No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA85590.30618059825950333No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG83970.30038538188866104No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG79750.2852892009720223No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT79450.2842160127551997No Hit
AGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGC78970.28249891160828344No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT76560.2738776329331415No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG75890.27148084591557087No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA74180.26536367307968173No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA73840.264147393100616No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA73350.26239451901313904No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC73210.2618936978452885No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG72780.260355461401176No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC70090.2507325403903329No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG69480.2485503910161268No Hit
TAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAAC69070.24708370045313585No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT66390.23749655238285344No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC64440.23052082897350618No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC64260.2298769160434126No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT63840.2283744525398609No Hit
ACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC63810.22826713371817864No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC62990.22533375259219673No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC62970.2252622067110752No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA62460.22343778674247666No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG61730.22082636208154155No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT61400.21964585504303663No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC59520.2129205422176147No Hit
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG59450.21267013163368936No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT59420.21256281281200712No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG59270.21202621870359578No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT58830.21045220931892256No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG57710.206445639976118No Hit
TGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGA57590.20601636468938894No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG57370.2052293599970523No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA57160.20447812824527645No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC55810.19964878126957453No Hit
CGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCC54900.19639344367854578No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA54850.19621457897574202No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG53230.19041936260489967No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG52510.1878437108845253No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA52440.1875933003006No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA51710.1849818756396649No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA51360.18372982272003846No Hit
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG50940.18222735921648675No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA50170.1794728427933086No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA49850.17832810869536445No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC49830.17825656281424293No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG48490.1734629887791017No Hit
AGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT47410.16959951119854016No Hit
GCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC47000.1681328206355492No Hit
ATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG45400.1624091501458284No Hit
TGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG45200.16169369133461328No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT44890.16058473017722988No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC44460.1590464937331174No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC44370.15872453726807062No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT44170.1580090784568555No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT43330.15500415144975208No Hit
TCGATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA43120.15425291969797622No Hit
AAATCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACA42840.15325127736227506No Hit
CTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCT42720.15282200207554603No Hit
TGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCA41500.14845770332713387No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC40810.1459893704284418No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG40680.14552432220115197No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA40010.14312753518358137No Hit
CCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGCTGG39990.14305598930245986No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC39890.1426982598968523No Hit
GAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG39070.13976487877087038No Hit
TGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGG37820.13529326120077598No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG37480.13407698122171033No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA37280.1333615224104952No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA36700.1312866918579714No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC36350.13003463893834497No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA36020.12885413189984007No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA35750.12788826250469967No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC35600.12735166839628834No Hit
AGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTC35340.1264215719417087No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC34970.12509797314096077No Hit
CGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTG34280.12262964024226865No Hit
GGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC34200.12234345671778263No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTG34160.1222003649555396No Hit
ACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA33930.12137758732264224No Hit
GGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGA33840.12105563085759542No Hit
AGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT33830.12101985791703469No Hit
AACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCTG33370.11937430265123994No Hit
TCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC33060.11826534149385654No Hit
CAGTGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGA32250.11536773330843536No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC32190.11515309566507083No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAAC32060.11468804743778101No Hit
CTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCATCC30790.1101448839865651No Hit
CTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTG30290.10835623695852736No Hit
CTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCC29970.10721150286058319No Hit
GGATCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGA29900.1069610922766579No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG29500.1055301746542277No Hit
GATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCA29240.10460007819964806No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA29170.10434966761572277No Hit
ATTCTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACG28700.10266833940936729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)