FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139308
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC1043172.0297868895968096No Hit
TTCCTCTGAGTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGA981911.9105879624260698No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT710441.3823650966239034No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT660061.284336334775032No Hit
AGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCC658461.281223075168875No Hit
GCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGGG597011.161654448419904No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC589021.146107608261657No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT582821.1340437272877983No Hit
AGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT452670.8807995161994572No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA451560.8786396923476858No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA407820.7935309578643662No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA375740.7311101027609165No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT354460.6897037499990272No Hit
GGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGGAG353300.6874466367845633No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC351790.6845084980312525No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC342850.6671131599818497No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA337370.6564502458307616No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG334640.6511382466277561No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC305690.5948077056288512No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG275570.5362005935429439No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA272210.5296627483700139No Hit
CGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCC252940.49216742798835955No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC243660.4741105222726484No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA243190.47319600226333974No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC240530.4680202081681036No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC228640.4448847977198487No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG211790.4120982824925068No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA199770.38870991970125157No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA181580.3533160495537532No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG177170.34473512776428267No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG176670.34376223413735857No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT175510.3415051209228947No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG174620.3397733702669698No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT169650.33010280761534433No Hit
ACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGC159100.309574752087246No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC158560.30852402697016795No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG157240.30595558779508836No Hit
AAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCCCCAACC156940.30537185161893393No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG156220.30397088479616324No Hit
AGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT155210.30200563966977656No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT152320.2963823145061553No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC148190.28834621314776226No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG146710.285466448012067No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC136180.26497730822904564No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC133740.26022958732965606No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA133680.26011284009442515No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA129850.25266047491218663No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG126000.2451691939848711No Hit
CGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT124570.24238671821186822No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG124160.24158894543779047No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG116680.22703445677900602No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA116070.22584752655415863No Hit
TGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGC114900.22357095546715627No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT114560.2229093878008479No Hit
TTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATC111010.21600184304968684Illumina RNA PCR Primer (96% over 30bp)
TCGATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA109960.21395876643314624No Hit
TCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA105710.20568917060429145No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA104580.20349043100744302No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC102250.19895674670597674No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC101800.19808114244174507No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA99710.19401444708120236No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC95740.18628967168342508No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC95530.18588105636011695No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG95360.18555027252696277No Hit
GGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC92270.17953778991257188No Hit
TGAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCC92100.1792070060794177No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA90440.1759769992380297No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC90410.17591862562041427No Hit
TGTCATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTC86930.16914728597702258No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG86620.16854409192832964No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT85990.1673182459584053No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG78820.15336695134831382No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC78620.15297779389754418No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCAATCTGAAGA77900.1515768270747735No Hit
AGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAA77630.1510514645162345No Hit
CCCTGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGA75460.14682910617538392No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA74570.14509735551945904No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG70590.13735312224914328No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG67750.13182708644821445No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG67240.13083473494875186No Hit
TGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG66370.12914190003790393No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC66010.1284414166265186No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC64830.12614538766697775No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT64030.1245887578638992No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT63800.1241412267955141No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT59130.11505440032004309No Hit
GGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTAC58640.11410096456565748Illumina RNA PCR Primer (95% over 22bp)
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC58580.1139842173304266No Hit
CCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG57200.11129903092011609No Hit
CAATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGC56860.1106374632538077No Hit
AAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGACCCA56700.110326137293192No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG55760.1084970972745747No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTG55650.10828306067665142No Hit
AGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG55220.1074463721574967No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA54480.10600648958964903No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA54220.10550058490364851No Hit
ACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC53720.10452769127672441No Hit
AATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACG52870.10287377211095346Illumina RNA PCR Primer (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA735150.0144.53712145
GGTCACA1199700.0144.4122145
CATCCTA232500.0141.39305145
AGTGCTG219800.0141.0191145
CGTGCTG171800.0140.66359145
AGTTGTA489200.0139.64526145
AGTGCTC421050.0139.62064145
CTTTCCA41100.0138.83612145
CGTACTG241850.0138.7748145
CGTTGTA117500.0138.16109145
GGTGCTC462550.0138.06644145
GAGTCAA140550.0137.973228
GAGAGCG105950.0137.939047
GAGCGAT104450.0137.145139
AGTCAAC140550.0137.096399
GCCTGGA452150.0136.55972145
AGAGCGA107400.0135.538098
CATTGTA77700.0134.65263145
TGAGTCA150000.0130.971167
TCTGAGT153000.0130.677415
Produced by FastQC (version 0.11.5)