FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5709934
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT964791.6896692676307643No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT836951.4657787638175852No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA714041.250522335284436No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC628041.0999076346591747No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT516180.9040034438226432No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA510810.8945987817022053No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA448620.7856833371454032No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG419590.7348421190157365No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG354040.6200421931321798No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC344330.6030367426313509No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT281720.4933857379087044No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG270210.4732278866971142No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC253430.44384050673790626No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC250160.4381136454466899No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA240260.4207754415375029No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG234810.411230672718809No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC225180.39436532891623616No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG219120.38375224652333983No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT215390.3772197717171512No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA206840.3622458683410351No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA206670.361948141607241No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG200530.3511949525160886No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG198600.3478148784206613No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT197230.34541555121302625No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG192810.33767465613437914No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA192320.3368165026075608No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG189730.3322805482515209No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA182250.3191805719645796No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC177100.310161203264346No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC174820.30616816236404837No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA168120.2944342263851036No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC161620.28305055715179894No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT159060.27856714280760514No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC156100.27338319497213104No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG154220.2700906875631137No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA149700.26217465911164645No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC144510.25308523706228475No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG143460.25124633664767404No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC140900.24676292230348024No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC140340.2457821754156878No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC135830.2378836603015026No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC133780.23429342615869114No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC130000.22767338466609244No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG126910.22226176344595228No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA124410.2178834291254505No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG121820.21334747476941066No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC118900.20823358028306455No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC116570.2041529726963569No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT115820.2028394724002064No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG114350.20026501181975134No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT113160.19818092468319248No Hit
CCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGG111730.19567651745186548No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG110530.19357491697802462No Hit
TCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGA109740.19219136333274606No Hit
CTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGG109670.192068769971772No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA108980.1908603496993135No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG107870.1889163692610107No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG106220.18602666860947956No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA105730.18516851508266122No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT103720.18164833428897778No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC102180.17895128034754867No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG97780.17124541194346554No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG97220.17026466505567314No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT95040.1664467575281956No Hit
CTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCA94730.16590384407245337No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC92500.16199836985856578No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG91170.15966909600005882No Hit
GAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTA90360.15825051568021628No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT89190.15620145521822146No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC88410.15483541491022487No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG87540.15331175456669024No Hit
CTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGC87210.15273381443638404No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG85750.150176867193211No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA82080.14374947241071437No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG82050.14369693239886835No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT81820.1432941256413822No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG77750.13616619736760532No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG77060.1349577770951468No Hit
ACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC76380.13376687015997032No Hit
CCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGA76240.13352168343802223No Hit
TCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGC76170.13339909007704817No Hit
CCAGAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTT75510.1322432098164357No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC75300.13187542973351354No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC74680.13078960282202912No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA74460.13040430940182496Illumina RNA PCR Primer (96% over 28bp)
TTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTAT73830.1293009691530585Illumina RNA PCR Primer (96% over 30bp)
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT73250.12828519559070212No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA73100.128022495531472No Hit
AAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGACCCA73060.12795244218234397No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA71730.12562316832383702No Hit
AAATCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACA71640.12546554828829895No Hit
CACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGG70740.12388934793291831No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT70640.12371421456009823No Hit
TGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAGC69120.12105218729323317No Hit
TAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAAC68100.11926582689046844No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA66770.11693655303196149No Hit
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG66580.11660379962360336No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC66530.1165162329371933No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG66490.11644617958806529No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT65990.11557051272396493No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCC65950.11550045937483691No Hit
TGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCA65900.11541289268842687No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA63890.11189271189474344No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC60330.1056579638223489No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC60320.1056404504850669No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC60300.10560542381050288No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC59330.1039066300941482No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC58090.1017349762711793No Hit
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG57700.10105195611718105No Hit
CCCTGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGA57260.10028136927677272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA784800.0144.33318145
GGTCACA764700.0142.17267145
CGTTGTA368000.0142.15079145
CATCCTA425600.0141.14049145
AGTTGTA621800.0139.81874145
GGTGCTC409100.0139.69077145
CGTACTG352300.0139.36864145
AGAGCGA179100.0138.715828
GAGAGCG182850.0136.864537
TGTTGTA132050.0135.01463145
CGTGCTG94100.0134.99112145
GAGCGAT179500.0134.973799
AGTGCTC718350.0133.16838145
CTACTAA30600.0126.747636
GGGTTGC66750.0126.200869
TTGACCA79950.0125.78207145
TGGGAGA188200.0125.26424
GCTGGGA194200.0121.765192
GGAGAGC203400.0121.715886
AATTCTA31400.0119.819132
Produced by FastQC (version 0.11.5)