FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5286991
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA1548452.928792577857613No Hit
ATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAG1126962.1315716255238564No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT934081.7667516362331617No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT597771.1306431200658371No Hit
AAGTCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACC596861.1289219141852143No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA518410.9805388357952567No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC501070.9477413523117403No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA498420.9427290494725639No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA466340.8820518135930249No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA436860.8262923088009796No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT404000.7641397535951925No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT368440.6968803237985463No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT284020.5372053782576894No Hit
TCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGC263780.49892273317658387No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG257010.48611771799876335No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC251130.4749960800008927No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC250950.4746556216948355No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT250210.47325595976993345No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG238220.4505776537164523No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC225670.4268401440441264No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA206910.3913568228128249No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC191270.361774778886516No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC183840.3477214165864856No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC180690.34176339623048346No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC177270.33529468841539545No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG171280.32396499256382316No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA164510.3111599773860027No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC162780.3078877947777857No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC149360.28250473662618303No Hit
TCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCAC146580.277246547232632No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT146440.2769817463279207No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT140550.2658411939797136No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC138870.2626635831231791No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT135780.2568190488691961No Hit
GCCCAGTGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTC125770.23788578418234493No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA122660.23200342122768886No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG121370.22956347003427846No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC119340.225723856915966No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC116590.22052241057342448No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT111010.20996820308564929No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG106320.2010973727778239No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA103960.19663358609840645No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC102600.19406123445264045No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG87820.1661058246552718No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG86280.16319301470344852No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG85350.16143398012215265No Hit
CCCCAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA85060.16088546396239375No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA83390.15772676745619577No Hit
TCCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTG81260.15369801083451817No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG80920.15305492292307668No Hit
GCTCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACA79810.15095543003572354No Hit
CCCGAATGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA78460.14840199274029406No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA75830.1434275186017907No Hit
ATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGC74740.1413658544151106Illumina RNA PCR Primer (96% over 29bp)
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA73770.13953116243246869No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC72480.1370912112390583No Hit
CCCTGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGA72370.13688315338535662No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG72000.1361833224229056No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC70960.13421622998790805No Hit
AGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG70350.13306245461738064No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC69210.13090621867901797No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC68300.12918501279839514No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC67780.1282014665808964No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA66420.1256291149351304No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG66190.12519408487739056No Hit
CAGTGATCGCTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGA65080.12309459199003744No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG62780.11874429141263906No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA61940.11715548598437184No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC61010.11539645140307597No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC60950.11528296530105688No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT60140.11375090292379919No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG59170.11191621094115727No Hit
CAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA58150.10998694720683276No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG57200.10819008392486387No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT56790.10741459556106678No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT56200.1062986488912124No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT55770.10548533182674227No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC55490.10495573001731988No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG54270.10264817927626507No Hit
TTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTA53650.10147548955540117Illumina RNA PCR Primer (96% over 30bp)
GATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAG53100.10043520028689286Illumina RNA PCR Primer (96% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA852800.0143.83734145
GGTCACA879300.0143.53534145
CATCCTA216400.0141.015145
CGTTGTA243000.0140.64943145
GGTGCTC325150.0139.92896145
GAGAGCG188850.0138.547127
AAGTCGC107700.0138.334961
AGAGCGA188200.0138.018848
GAGCGAT184950.0137.895029
AGTGCTC958600.0137.81175145
GTCGCTT114550.0137.654943
AGTCGCT115650.0135.283782
CGTACTG130000.0134.54909145
AGTTGTA367050.0134.20949145
CGTGCTG115800.0133.88933145
CTTTCCA33600.0133.81328145
TGTCTTG563950.0131.13579145
TGGGAGA187650.0130.279114
TGTTGTA77000.0130.15598145
GCTGGGA188250.0129.557042
Produced by FastQC (version 0.11.5)