FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564418
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT714752.7871821208554923No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC677092.6403261870724664No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA494731.9292096686265656No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC455721.7770893824641691No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA384561.4995995192671399No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT337011.3141773299048751No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT293241.1434953272048474No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA288161.1236857641772908No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC272781.0637111422552799No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG267011.0412109102338232No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA239480.9338571168974793No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC183170.7142751298735229No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC164480.6413930958213521No Hit
ACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGC161080.628134726865901No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG148630.5795856993672638No Hit
AGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGC145260.5664443160202431No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT144200.5623108245223672No Hit
CTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCA142050.5539268559181849No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC130320.5081854830218786No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA126830.49457615724113624No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG122470.47757424881591065No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT116190.4530852614511363No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC115230.4493417219813618No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG114960.4482888515054878No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT110390.43046804382124915No Hit
CTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGGAGT107080.41756063169108937No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG102460.3995448479928No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA97180.3789553809090406No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG94390.36807571932500865No Hit
AGAGATGCCTGATGCAACTTTAGCCACTCTGAAGATCCAGCCCTCAGAAC94230.3674517960800462No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG93230.3635522757990312No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG89230.3479541946749711No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT87520.3412860149944354No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT84500.32950946374577No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG84310.3287685548923771No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC83560.3258439146816159No Hit
AAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACACAGCAGG83150.3242451113663997No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT81750.31878578297297866No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC79830.3112987040334298No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC79080.3083740638226685No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA75810.2956226325037494No Hit
TCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGA74010.28860349599792234No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA73520.28669273106022497No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC68590.2674680960748209No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA63580.24793149946693557No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG63200.24644968176014986No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT62600.24410996959154085No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT59830.23330829841312922No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC59320.23131954306981156No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC56590.22067385270264053No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA56270.2194260062127157No Hit
TCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGC55220.21533150991764993No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA53730.20952122469893755No Hit
TGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCACAC53250.20764945496405032No Hit
ACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC52980.20659658448817625No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG52340.20410089150832667No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC52130.20328199224931348No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA51930.20250208819311047No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG51290.20000639521326086No Hit
TTACTTTAACAACAACGTTCCGATAGATGATTCAGGGATGCCCGAGGATC49240.19201237863718004No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG48880.19060855133601465No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG48530.1892437192376594No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG47560.18546118456507482No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC46960.18312147239646578No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC46650.18191262110935114No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC46350.18074276502504663No Hit
CGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCC45250.17645329271593008No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC44960.17532243183443572No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG44440.1732946812883079No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA43970.17146190675623085No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA43520.16970712262977408No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA42990.16764037688083613No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG41940.16354588058577035No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG41480.16175210125650344No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA41220.1607382259834395No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCGCA41210.16069923078062937No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT40800.15910042746541322No Hit
TGAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCC40770.15898344185698274No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA39670.15469396954786624No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG39030.1521982765680166No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC38790.15126239170057298No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA37860.147635837839229No Hit
ATTCTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACG37610.14666095776897525No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG36600.14272244228515008No Hit
TAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC36020.14046072052216135No Hit
TCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC35490.1383939747732234No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT35450.1382379939619828No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG35380.13796502754231174No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG35320.13773105632545085No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT35120.13695115226924784No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC34200.133363593610714No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC33320.12993201576342078No Hit
CAGTGATCGCTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGA33140.12923010211283809No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC33060.12891814049035688No Hit
AAATCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACA32860.12813823643415387No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG31670.12349780729974599No Hit
CCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCTTGGAGCTGG31560.12306886006883432No Hit
TGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGG31530.12295187446040387No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTG31150.12147005675361816No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC30920.12057316708898472No Hit
TGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAGTGC30510.11897436377376854No Hit
CCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGCTGG30360.1183894357316163No Hit
CATCTGTCTTCTGGGGGCAGGTCTCTCAAATGCCGGCGTCATGCAGAACC30100.11737556045855238No Hit
ATTTTCAGGTCGCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACG29700.11581575234614638No Hit
AGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCTTGG29560.11526981950680426No Hit
TCGCTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCC29550.11523082430399412No Hit
AGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG29550.11523082430399412No Hit
AAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC29430.11476288187027231No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGCTC28690.11187723686232119No Hit
CTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCCAAA28620.11160427044265016No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC28380.11066838557520653No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG27750.10821168779816706No Hit
AAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACA27250.10626192765765954No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA26970.10517006197897534No Hit
CATTCTTGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAAC26770.10439015792277234No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG26270.10244039778226482No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG25980.10130953690077046No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT25910.10103657048109942No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG25720.10029566162770656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA224450.0144.05841145
CGTACTG224150.0143.18413145
AGTTGTA346250.0143.01582145
GGTCACA458850.0142.451145
CATCCTA132050.0140.07747145
CGTGCTG64850.0138.70363145
GGTGCTC196200.0138.5346145
AGAGCGA60700.0138.077278
GAGCGAT60450.0136.364189
TGTTGTA39250.0135.72932145
AGTGCTC389800.0135.49803145
CGTTGTA184050.0133.62079145
GAGAGCG63150.0133.526647
GCCTGGA55150.0130.90005145
GGGTTGC14150.0130.653159
TAACAAC7100.0128.668957
AGGAAAA5850.0125.1728441
AGTGCTG62500.0125.01576145
TGGGAGA62850.0124.6995854
GCTGGGA63900.0122.875052
Produced by FastQC (version 0.11.5)