FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4306645
Sequences flagged as poor quality0
Sequence length109-123
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA1552253.6043137987923317No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT988762.2958939034910006No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA701401.6286459645501312No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT661811.5367182574834934No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT467721.0860426155394745No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC407610.9464676099376661No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT382830.8889286207709248No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG317850.7380455087428845No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC313910.7288968559052349No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG303420.7045391482232689No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC275730.6402431591180605No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG268920.6244303860661837No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC235990.5479671530855225No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA220740.5125567582189848No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT196180.45552860753556423No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG194260.4510703807720394No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG193620.4495843051841979No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC179450.4166816628721429No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC166860.3874477696675719No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG148040.34374785941260544No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG146960.3412401068581227No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATCTG145050.33680510002565806No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT135180.31388702806941365No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC134920.31328330986185304No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT134480.3122616328952119No Hit
GACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGT133200.30928948171952875No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG128690.29881729281145764No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT116080.2695369597447665No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC114520.26591465049940266No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG113410.2633372381517399No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC111410.25869325193973497No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT111040.2578341144905141No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT105910.24592228985672143No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG105160.2441807950272196No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA100470.233290647360068No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC100120.23247794977296712No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC99130.23017917659802467No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT93810.21782617327409157No Hit
CAGGGCCAGGGTAGTGATGGGGGCTGTGGCTTCTCTATAAGGACATGCCC91710.21294998775148638No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG85850.1993431081503119No Hit
GAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGAGGACCTGAAAAA85200.19783381263141028No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG85080.19755517345868998No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG83210.1932130463504654No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC82790.19223780924594436No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG82650.191912730211104No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA81190.1885226202763404No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG79320.18418049316811577No Hit
GACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGGA77250.17937396743869066No Hit
GAGTGCTGGGTAAGCTGGGGCCGCCGGGGGACCGGGGACGAGACTGCGCT76080.1766572355046678No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG75240.1747067612956257No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC74720.17349932488050443No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA73340.170294974394221No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC72100.16741570294277797No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTACCAATCTG70670.1640952528011944No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT69420.16119276141869135No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG69180.16063548307325073No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT69060.16035684390053045No Hit
GACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGA68210.15838314976042836No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT68040.15798841093240795No Hit
AGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAG68030.15796519100134793No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC67240.15613081644760596No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC67180.1559914968612458No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG67140.15589861713700573No Hit
GGCTCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCA66260.15385526320372356No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT66030.153321204789343No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCA66000.15325154499616292No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC63880.14832891961143768No Hit
GCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC63770.1480735003697774No Hit
GTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT61430.14264003650173163No Hit
GACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATC60880.14136294029343027No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA60650.14082888187904968No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT60100.13955178567074833No Hit
GACTGGTATCGGCAGCTCCCAGAGGAAGGTCTGAAATTCATGGTTTATCT59360.13783351077230652No Hit
GAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAA59150.137345892220046No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACC59050.13711369290944575No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG58810.13655641456400516No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT57130.132655466145921No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC55430.1287080778657168No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG54850.12736132186423538No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG54580.1267343837256147No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC54330.1261538854491141No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC54220.12589846620745382No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC54030.12545728751731336No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG53800.12492322910293278No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT52560.12204395765148973No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC52140.12106872054696871No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG51680.12000060371820756No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT51350.11923434599322674No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC50910.11821266902658566No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC50800.11795724978492539No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC50560.1173999714394848No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC50340.11688913295616425No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA49920.11591389585164322No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT48830.11338292336610054No Hit
GCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAA47880.11117702991539817No Hit
GGAGAAGGAAGCCCCCGGCCTGGTCCATACCCCACCACCAACTTGCATAA47020.10918011584423606No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT46920.10894791653363581No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC46340.10760116053215438No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC46310.1075315007389743No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG46040.10690456260035364No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC45180.1049076485291915No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT44810.10404851107997061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCAA19100.097.768061
GGATCTG32950.096.698011
GCATGCT9150.095.026671
AGTCATT16500.094.774364
GTCTTTC15250.094.5164641
GATGCTC136350.094.327171
GAGTGCT11600.093.569721
GCTTTTC37250.093.055181
AGTGGTA307200.092.483372
GAGTGAT38150.092.236571
CATTGAG15850.091.663197
ATTGAGG15250.091.443538
GATTCCT24200.090.426611
GCTATTT6300.088.922361
GACTGGT9300.087.84671
GATGTTC97150.086.4967961
GAGAACT13900.086.063581
AATGCTT15450.084.597894
GATCCCT3300.083.112111
GCTATTC4700.081.9457551
Produced by FastQC (version 0.11.5)