FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4306645
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT457751.06289234427263No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC321220.7458706255101129No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT295770.68677590096235No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA264030.6130758397778318No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA254610.5912026647192884No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT245560.5701886271099661No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC219630.5099793458713221No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA215730.5009235727579124No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG213750.4963260264080276No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA207000.48065257294251096No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC188260.43713842213602466No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC167290.38844622670315293No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT146410.3399630106498214No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA125130.29055099735408885No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA120160.2790106916172566No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA116880.2713945542295685No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG113620.26382485670400047No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC112840.2620137020813185No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA111260.25834495297383464No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC110770.25720717635189344No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC109310.2538170664171298No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG107510.24963747882632537No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG105400.24473807337266015No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC102670.23839903219327344No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG100100.23243150991084705No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG94920.2204035856217543No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG90160.20935089843718255No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT87330.20277965794719555No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC85930.1995288675987921No Hit
TTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTAT82930.1925628882807847Illumina RNA PCR Primer (96% over 30bp)
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG82810.19228424910806438No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT81580.18942819758768137No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC81490.18921921820814114No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC80070.18592198799761764No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG77140.1791185481970304No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA75940.17633215646982744Illumina RNA PCR Primer (96% over 28bp)
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC73730.17120055170556198No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG71540.1661153868034166No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT68070.15805807072558803No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC65240.15148683023560103No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG62680.14554252788423472No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA60600.14071278222374958No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA59540.13825146953138698No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT59370.13785673070336654No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG58860.13667251421930526No Hit
CGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTA58460.1357437169769043Illumina RNA PCR Primer (95% over 23bp)
ATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGA57330.1331198647671215Illumina RNA PCR Primer (96% over 26bp)
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC56030.1301012737293183No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT55800.12956721531493773No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC55770.12949755552175765No Hit
TTTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTG54530.1266182840703146Illumina RNA PCR Primer (96% over 30bp)
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC53510.12424985110219208No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC53310.12378545248099158No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG53270.12369257275675147No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT52870.1227637755143505No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG52850.12271733565223045No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA52850.12271733565223045No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT52550.12202073772042972No Hit
CTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGG52400.12167243875452934No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG51660.11995416385608751No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA51640.11990772399396747No Hit
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG51370.11928078585534681No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC50240.11665693364556401No Hit
GGGGGCTGTGGCTTCTCTATAAGGACATGCCCCAACGTGACAACAGCTTG49940.11596033571376325No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA49900.11586745598952317No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG48660.1129881845380801No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC48550.11273276529641983No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT48140.11178074812295882No Hit
TAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAAC47860.11113059005327813No Hit
GGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTAC47840.11108415019115808Illumina RNA PCR Primer (95% over 22bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTTTTTTTTTTAATGATA47350.10994637356921688No Hit
ACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC47290.10980705398285673No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG46770.10859961756773545No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG45830.10641694404809313No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG45300.10518628770191181No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT44570.10349123273453002No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT44060.10230701625046874No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC43940.10202837707774845No Hit
AAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGACCCA43890.10191227742244832No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA43880.10188905749138831No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG43610.10126211935276763No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA43550.1011227997664075No Hit
TGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGA43330.10061196128308696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA658900.0144.3746145
GGTCACA703000.0142.26382145
CATCCTA270550.0140.68744145
GCCTGGA539450.0140.14934145
CGTTGTA146750.0139.52545145
GGTGCTC379050.0138.98709145
AGTGCTC511550.0136.82918145
AGAGCGA101350.0135.247288
CGTACTG226050.0134.96962145
AGTTGTA257900.0131.0738145
GAGAGCG105500.0131.026467
GAGCGAT101800.0130.805869
CGTGCTG67000.0130.27628145
CTTTCCA28600.0129.63713145
AGTGCTG138450.0126.82268145
TGTTGTA70550.0125.26358145
CTACTAA11900.0116.346216
TGGGAGA111850.0114.516064
GCTGGGA111850.0114.25552
GGAGAGC123000.0110.558876
Produced by FastQC (version 0.11.5)