FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362704
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC377951.5996502312604541No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT325411.3772778985433638No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA243261.029583053992375No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC228650.9677471236346152No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA221370.9369349694248623No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT201610.8533019794269616No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT189040.8001002241499571No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG175470.7426660301078765No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA154550.6541234111424876No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA145770.6169625987851207No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC133970.5670198213572246No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA116830.49447582092382286No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG113080.47860417555478807No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC103980.440088982792597No Hit
GGATCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGA92410.39111966628066824No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG89330.37808375488423435No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC87910.3720736918378265No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC87770.37148115041071583No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT82830.3505729029112407No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA80150.33922996702083713No Hit
TTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTAT73920.312861873514414Illumina RNA PCR Primer (96% over 30bp)
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG72790.3080792177098782No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT68290.28903324326703644No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC68190.2886099993905288No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA66870.28302318022062856No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT65580.27756333421368057No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA64880.27460062707812743No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC64600.2734155442239062No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG64220.2718072174931773No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG63950.2706644590266068No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC63710.26964867372298856No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT62910.2662627227109278No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG62460.26435812526664365No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG59710.2527189186626848No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT59130.25026410417894074No Hit
ATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGA58980.24962923836417936Illumina RNA PCR Primer (96% over 26bp)
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA57670.24408474358192986No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA56320.2383709512490773Illumina RNA PCR Primer (96% over 28bp)
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC52950.2241076326107714No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC52620.22271092781829632No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC51730.21894405731737876No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC50990.21581205263122255No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT50830.2151348624288104No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG48400.20485003622967582No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT47790.20226824858297948No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG47760.20214127542002724No Hit
CGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTA45080.19079833952962366Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC44740.18935931034949788No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG44470.18821655188292735No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA43840.1855501154609295No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA43600.18453433015731127No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC43110.18246043516242405No Hit
ACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGT42660.1805558377181399Illumina RNA PCR Primer (95% over 24bp)
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG42030.17788940129614203No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG41690.17645037211601625No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG41590.17602712823950864No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC41430.17534993803709648No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA41100.17395323324462142No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA40990.17348766498046306No Hit
TTTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTG40840.17285279916570168Illumina RNA PCR Primer (96% over 30bp)
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG40800.17268350161509863No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC40540.17158306753617888No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC40510.17145609437322662No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT40430.17111749927202052No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG40240.17031333590665612No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA40150.16993241641779927No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA39780.16836641407472117No Hit
TTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGG38520.16303354123072547Illumina RNA PCR Primer (96% over 30bp)
TTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAA38420.1626102973542179Illumina RNA PCR Primer (96% over 30bp)
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC38370.1623986754159641No Hit
TGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAG38110.16129824133704435No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC37810.16002850970752155No Hit
GGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTAC37750.159774563381617Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT37220.15753137083612676No Hit
GGGGGCTGTGGCTTCTCTATAAGGACATGCCCCAACGTGACAACAGCTTG37100.15702347818431764No Hit
TTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTA37050.15681185624606384Illumina RNA PCR Primer (96% over 30bp)
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG36950.15638861236955623No Hit
CGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCC36280.15355287839695536No Hit
ACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC35940.15211384921682952No Hit
AATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACG35740.15126736146381436Illumina RNA PCR Primer (96% over 30bp)
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT35490.15020925177254535No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT35000.14813535677765813No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC34750.14707724708638914No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG34650.14665400320988156No Hit
ATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGC34620.14652703004692927Illumina RNA PCR Primer (96% over 29bp)
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC34560.14627308372102474No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC33890.14343734974842384No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA33640.14237924005715485No Hit
TTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGT33460.14161740107944118Illumina RNA PCR Primer (96% over 30bp)
ACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA33080.14000907434871232No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG31620.13382971375170144No Hit
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG30840.1305284115149422No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG30590.12947030182367322No Hit
TAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAAC30480.12900473355951486No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC30450.1288777603965626No Hit
TAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAAC29840.12629597274986626Illumina RNA PCR Primer (96% over 30bp)
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC29720.12578808009805714No Hit
AGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGC29150.12337559000196385No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC28160.11918547562453866No Hit
TTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCA27510.1164343904272393Illumina RNA PCR Primer (96% over 30bp)
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG27360.1157995246124779No Hit
TTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATC26460.11199032972390956Illumina RNA PCR Primer (96% over 30bp)
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG26210.11093222003264057No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC25990.11000108350432386No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA25770.10906994697600714No Hit
TCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC25170.10653048371696157No Hit
AAATCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACA25010.10585329351454943No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC24760.10479518382328044No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT23900.10115528648531513No Hit
ATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG23830.1008590157717598No Hit
AGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA23760.1005627450582045No Hit
AGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT23650.10009717679404614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)