FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19338551
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT2700281.3963197139227235No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1738460.8989608373450524No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC1360090.7033050201124169No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT1326300.6858321494717986No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA1197090.6190174227634739No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA1167090.6035043680366745No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG910880.471017709651566No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG898800.4647711196149081No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT874170.4520349016842058No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT853890.44154807668888946No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC786830.4068712283562507No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA706680.3654255171444851No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA581900.30090155151748443No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA579100.2994536664096498No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC531730.27495855299603367No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG524320.2711268284785142No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA515240.2664315439145363No Hit
TTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTAT493650.25526731552948306Illumina RNA PCR Primer (96% over 30bp)
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA489140.2529351863022209No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG484800.25069096438507726No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC462430.2391233965771272No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC451780.23361626214911344No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG435800.22535297499797166No Hit
GGATCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGA408660.2113188314884605No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT405520.20969513176038887No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG401850.20779736806547708No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC399770.2067217962710857No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA396060.20480334850320484No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA387750.2005062323438814No Hit
TGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCA374040.1934167663337341Illumina RNA PCR Primer (96% over 28bp)
ACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGT356130.1841554726618349Illumina RNA PCR Primer (95% over 24bp)
ATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGA355670.18391760582269065Illumina RNA PCR Primer (96% over 26bp)
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT355250.18370042305651546No Hit
TTTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTG351750.1818905666717222Illumina RNA PCR Primer (96% over 30bp)
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG349000.18046853665509893No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG340200.17591804060190444No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC337820.17468733826024505No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA331680.17151233305949345No Hit
CGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTA328640.16994034351384446Illumina RNA PCR Primer (95% over 23bp)
GGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGCAGAGTAC322860.16695149496981443Illumina RNA PCR Primer (95% over 22bp)
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT314770.1627681412118209No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC313890.16231309160650145No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT301090.15569418825640038No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC294250.15215721177869015No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG290420.1501767117919021No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC285390.1475756896160421No Hit
AATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACG263440.1362253045742672Illumina RNA PCR Primer (96% over 30bp)
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC261990.13547550692913857No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG256820.13280209049788683No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC253320.13099223411309358No Hit
TTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTA251590.13009764795718148Illumina RNA PCR Primer (96% over 30bp)
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC251050.12981841297209912No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT248840.1286756179405582No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC244800.1265865265706826No Hit
ATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAACGC244800.1265865265706826Illumina RNA PCR Primer (96% over 29bp)
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC244090.12621938427548166No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA241500.12488009055073464No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT235170.12160683600337999No Hit
TAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAAC233250.12061400050086483Illumina RNA PCR Primer (96% over 30bp)
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG228300.11805434647094294No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC225900.11681330209279898No Hit
TTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCAA224020.11584115066325289Illumina RNA PCR Primer (96% over 30bp)
GGGGGCTGTGGCTTCTCTATAAGGACATGCCCCAACGTGACAACAGCTTG221010.1142846741723307No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA220090.11380894049404218No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC217720.11258340917062505No Hit
TTTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGG215630.11150266635799136Illumina RNA PCR Primer (96% over 30bp)
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG214430.11088214416891938No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA213010.11014785957851754No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA211760.10950148229823423No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC210680.10894301232806947No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTTTTTTTTTTAATGATA210110.10864826428826027No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT208660.10789846664313164No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG205630.10633164811572492No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC203010.10497684133625111No Hit
TTTTTTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGT197110.10192594057331389Illumina RNA PCR Primer (96% over 30bp)
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC194530.10059181786680915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA2785100.0144.27896145
GGTCACA2117800.0142.32124145
CATCCTA1144900.0141.16548145
CGTTGTA1104400.0140.96982145
AGAGCGA577850.0138.375998
CTTTCCA260100.0137.98479145
GGTGCTC1374000.0137.59824145
AGTGCTC2419550.0137.41463145
GCCTGGA2691050.0136.56146145
GAGAGCG598400.0134.726277
GAGCGAT581800.0134.584089
TTGACCA405850.0132.18228145
AGTTGTA1689200.0128.80246145
TGTTGTA310700.0122.16628145
TGGGAGA610550.0120.351174
GCTGGGA616750.0119.599072
GGAGAGC685200.0115.8292166
CGTACTG985400.0115.75709145
AGGATCG135250.0114.4254763
GGGTTGC198950.0112.985079
Produced by FastQC (version 0.11.5)