FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745801
Sequences flagged as poor quality0
Sequence length117-124
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT316954.24979317539129No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC264363.5446452874158116No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT252993.392191750882608No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT176892.371812319908394No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT160922.1576801318314134No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC159732.1417241328450887No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA118271.58581176480053No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC116321.5596653799069726No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG116041.5559110272043077No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG95471.2801001875835512No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG90821.2177511159142989No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA87751.176587320210083No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT85991.1529885317933335No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG72780.9758635346426191No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT61250.8212646537078925No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG53080.7117180052051418No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT52050.6979073506203397No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT51230.6869124605625362No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG50840.6816831835838246No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG47190.632742514424089No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC43330.5809860807373549No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG40610.5445152259114696No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT40530.5434425537107084No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC38430.5152849084407235No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA36410.48819993537149986No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG35280.47304844053574613No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC33080.44354995501480965No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC31020.4159286458452054No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC29730.39863180660792896No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC28610.3836143957972703No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT28470.38173721944593797No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC26310.3527750700253821No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT25580.34298693619343495No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC25570.34285285216833983No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG25360.34003708764134133No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC25350.33990300361624615No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC24750.3318579621105362No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG24300.3258241809812537No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC23470.3146952068983549No Hit
GAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCT23190.3109408541956903No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG22150.296996115585793No Hit
AGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT21620.2898896622557492No Hit
ACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG21590.2894874101804637No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT21300.28559897345270385No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA20850.2795651923234214No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT20840.27943110829832624No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC20110.2696429744663791No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG19890.26669312591428546No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA19750.2648159495629531No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG19470.26106159686028846No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC18560.24885995057662835No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC18000.24135124517129905No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG17930.2404126569956329No Hit
GCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT17840.2392059007697764No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA17660.23679238831806343No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA17140.22982001901311477No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG17110.22941776693782925No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC17070.22888143083744858No Hit
CAGGGCCAGGGTAGTGATGGGGGCTGTGGCTTCTCTATAAGGACATGCCC16780.2249929941096888No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG16740.2244566580093081No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT16360.21936146505569182No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG16320.21882512895531114No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG15740.2110482554997915No Hit
GGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC15380.20622123059636555No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT15000.2011260376427492No Hit
GCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC14720.19737168494008456No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT14360.19254466003665857No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT14200.1903993156351359No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG13890.18624271085718577No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC13840.18557229073170992No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG13570.18195202205414043No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC13530.1814156859537598No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG13500.18101343387847427No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA13360.17913625752714196No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC13160.17645457702523865No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT13090.17551598884957248No Hit
GCACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGA12850.1722979722472885No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC12620.16921403967009968No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT12540.16814136746933833No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC12520.167873199419148No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC12450.16693461124348183No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC12230.16398476269138818No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT11690.1567442253362492No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC11650.15620788923586856No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT11590.15540338508529755No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT11530.15459888093472654No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC11400.1528557886084894No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG11220.1504422761567764No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC11190.15004002408149092No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT11140.1493696039560151No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC11060.14829693175525374No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT10980.14722425955449242No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG10770.14440849502749392No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT10740.14400624295220843No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC10610.14226315062597128No Hit
GAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGGCC10610.14226315062597128No Hit
GGAGCCCCACCAGGGAGACCCAGTGACATAGATGGTCTGCTCAGGGTGAT10520.14105639440011478No Hit
AGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCTG10420.13971555414916312No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC10210.13689978962216462No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT10160.1362293694966888No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT10030.13448627717045164No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG9860.13220684874383382No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC9640.12925700019174016No Hit
GGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTC9590.12858658006626433No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC9550.12805024396588366No Hit
AGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAG9440.12657531968983685No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG9400.12603898358945617No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT9340.12523447943888516No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG9240.12389363918793352No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT9090.12188237881150601No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT8840.11853027818412687No Hit
TGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCTGGGGCCAG8780.11772577403355586No Hit
GGTGTCTCCGCAGATCACACAGGTGCTGGAGTCTCCCAGACCCCCAGTAA8710.1167871858578897No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC8660.11611676573241388No Hit
ATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCTG8620.1155804296320332No Hit
GGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG8590.11517817755674771No Hit
GCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC8580.11504409353165254No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC8480.11370325328070088No Hit
GAGAGTGGGAAGGAACTACGCTGTAGGAAGGAAAGATGAACTTGAGTTTC8460.11343508523051055No Hit
GGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT8410.11276466510503473No Hit
GTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT8410.11276466510503473No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC8410.11276466510503473No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA8160.10941256447765557No Hit
GCTATTTCCCCAGGCAGGGCTGGGAGAGATGAGATCCTGGCCTGGACCTG8110.10874214435217973No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA8060.1080717242267039No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT8030.10766947215141841No Hit
GACAGTCATGTTTACTGGTATCGGCAGCTCCCAGAGGAAGGTCTGAAATT7990.10713313605103775No Hit
GGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGT7980.10699905202594259No Hit
GGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCCTTCTGTCTCC7800.1045855395742296No Hit
GAGTGCTGGGTAAGCTGGGGCCGCCGGGGGACCGGGGACGAGACTGCGCT7700.10324469932327793No Hit
GTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTC7660.10270836322289725No Hit
TGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCC7580.10163569102213593No Hit
GCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCG7500.1005630188213746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)