FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3692294
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC622741.6865937544518392No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA468051.267640117498769No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT449841.2183211846077264No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC444311.2033440457341695No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA443861.2021252912146216No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT406401.1006707483206917No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA397001.0752123205790223No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG374251.0135975087574283No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA368040.9967786963876658No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC340080.9210534155730827No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG323060.8749574113004002No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG260310.7050088644078722No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT248110.6719670752112372No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC228950.6200752161122597No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG227440.6159856176133319No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA208380.5643645928520318No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT208090.5635791732727675No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC202620.5487645350018172No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC200010.5416957587884389No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA199480.540260336798749No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA194270.5261498678057598No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC193140.5230894397900059No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC191600.5189185909897749No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA184270.4990664340380262No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA161280.4368016198060068No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG157670.42702450021585503No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG154460.4183307179764125No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT152370.4126702803189562No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG151500.4103140215811633No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG139230.3770826483481543No Hit
AGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG123760.33518457630947046No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC120640.3267345449739376No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA119570.32383661756079013No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC118300.320397021472288No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT116820.3163886732746634No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC114060.3089136455547689No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC111200.30116778349719714No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT108200.2930427533668771No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT106750.2891156554705557No Hit
TTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGG105710.28629897835871143No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG104750.28369896871700895No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG104400.2827510485351383No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG102660.2780385310595527No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA101660.27533018768277934No Hit
ACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGG101580.27511352021263746No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC99870.270482253038355No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG97620.26438848044061497No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT97340.26363014429511844No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT97000.2627093075470155No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC96260.2607051334482032No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT96190.2605155494118291No Hit
TCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA93760.2539342750062698No Hit
CTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGC92950.2517405168710834No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC92780.2512800984970319No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC92090.2494113415670583No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC91750.24849050481895538No Hit
GAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTA89900.24348006957192464No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG88470.23960713854313878No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC85310.23104877347253494No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG84580.2290716828074904No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC83500.2261466719605752No Hit
AGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGC83000.22479250027218853No Hit
TCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGA82830.22433208189813705No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG81600.22100081954470582No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA81380.22040498400181566No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC80910.2191320626147322No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA80220.2172633056847586No Hit
GAAGGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGA79190.214473712006682No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC78120.21157578459353454No Hit
CCAGAATCTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCTGAGTT77670.2103570300739865No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG76410.20694451741925213No Hit
TGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGA75900.20556326229709768No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG75330.2040195065723369No Hit
ATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT74750.20244866741380832No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG73850.20001115837471228No Hit
TGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG73800.19987574120587365No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC73560.19922573879544805No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA72600.1966257291537456No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA70870.1919402951119277No Hit
TCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCC70550.19107362523136023No Hit
ACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCC70180.1900715381819541No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA70150.1899902878806509No Hit
TGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGC69410.1879861137818386No Hit
GCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC68960.1867673592622906No Hit
AGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA66950.18132358907497614No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC66740.18075483696585376No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC66140.17912983093978974No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC64620.17501314900709425No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG64300.17414647912652675No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC62890.17032771496527632No Hit
ATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAG61230.16583186495983257No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA60700.16439644297014266No Hit
TCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCA60080.1627172700765432No Hit
TGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG60000.16250060260640134No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG59170.16025267760367945No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA58060.157246416455461No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC57620.15605474536968073No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT57440.15556724356186152No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT56550.15315681795653324No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG56100.15193806343698524No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT55700.1508547260862759No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT55290.14974430530179883No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC55120.14928388692774736No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA52540.14229636101567208No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA51960.14072552185714354No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT50910.13788176131153154No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG48900.13243799112421709No Hit
TGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCA48690.13186923901509467No Hit
TAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAAC48650.13176090528002374No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT48410.13111090286959814No Hit
GAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACACAGCAGG48080.13021714955526292No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA47980.1299463152175856No Hit
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG47740.12929631280716No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG46950.12715672153950905No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG46670.1263983853940125No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC45660.12366295858347143No Hit
GGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGA44770.12125253297814312No Hit
ACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG43870.1188150239390471No Hit
TCCTGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGA43400.11754210255196364No Hit
ATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG43110.11675668297269935No Hit
TGGCTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGG42800.11591709652589961No Hit
ACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGC42690.11561917875445454No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC41970.11366917152317772No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT41760.11310041941405534No Hit
CTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTACCA40600.10995874109699824No Hit
CAGTGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGA39900.10806290073325689No Hit
TGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCGCTA39630.10733164802152809No Hit
ATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCAGCCAG39310.1064649781409606No Hit
CGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCC39090.10586914259807048No Hit
TGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAA38850.10521914018764486No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG38800.1050837230188062No Hit
ACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA38680.1047587218135934No Hit
CTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTC38550.10440663717461286No Hit
AGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTC38290.10370246789665179No Hit
CAATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGC37800.10237537964203285No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC37700.1021045453043555No Hit
CTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCC37390.10126495885755576No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG37300.10102120795364615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)