FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3040705
Sequences flagged as poor quality0
Sequence length116-123
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT1390544.573084202512246No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT1057133.4765950659468774No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC943283.1021753178950275No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG858412.8230624148018304No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA593711.9525406114700372No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA491271.6156450559985265No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG484381.592985837166052No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG483901.5914072558830927No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC465741.531684264011142No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG438741.4428890668446956No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG372461.2249133013561No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG306531.0080885847196621No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT232640.7650857284741532No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC228480.7514046906885081No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT220520.7252265510794372No Hit
GATTCCAGCCTCTGACAAACTCTGCACATTAGGCCAGGAGAAGCCCCCGA214820.7064808983442985No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT214520.705494285042449No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC207370.6819800013483714No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT207300.68174979157794No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC183960.6049912766940562No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT177260.5829569129527528No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA176950.5819374125408416No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC163420.5374411526274335No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG162060.5329685056590494No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT161150.5299757786434396No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC160360.5273776969485695No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT139070.4573610396273233No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC136830.4499943269735143No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC136290.44821842303018544No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA136060.44746201949876757No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC129420.42562497841783403No Hit
CAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG127530.41940931461618275No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG125900.4140487156761343No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT119630.3934284976674817No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT118120.38846254404817304No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT115100.3785306368095557No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA114730.37731381373727474No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT106920.3516289807791285No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT105570.3471892209208062No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG102150.33594182927972294No Hit
GACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTG96140.3161766761326732No Hit
GAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGA94510.3108160771926247No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT93580.30775757595689157No Hit
GACCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGACTCTG92250.30338359031869255No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT89430.2941094252813081No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT84770.27878403199258067No Hit
ACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCTT84470.27779741869073127No Hit
GAGTCCTGACCTGCCTGACTCTGCCTGGAACACCAGGCTCCTCTGCCATG84140.27671214405869693No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC79830.26253779962212714No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC79150.26030147613793514No Hit
GGCAAAAGCCCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGA78500.25816381398392807No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC78440.2579664913235582No Hit
GCACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGA78060.2567167811412156No Hit
GGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGAC77270.2541186994463455No Hit
GAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCTTCCAA77180.2538227154557907No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT75600.24862655206605047No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG73640.24218067849396768No Hit
GAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTG72500.2384315479469399No Hit
GAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAG71890.2364254342331795No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA68300.22461896172104823No Hit
GGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGTCTTGATTATCTCC66650.2191925885608765No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC66440.2185019592495819No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG66090.2173509103974243No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC65810.2164300713156982No Hit
GGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTC65760.21626563576538993No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG63360.20837272935059467No Hit
GACAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGT62680.20613640586640267No Hit
AGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGAC62480.20547866366516973No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG61500.20225572687912835No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA60580.19923011275345687No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT59960.19719111192963473No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC59730.19643470839821683No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC59190.19465880445488792No Hit
GACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGA58980.19396817514359335No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT58500.19238959386063428No Hit
GACACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCT58180.1913372063386616No Hit
GAGAGGTAAGCAGCTGCCCCACTCTGAGAGGGGCTGTGCTGAGAGGCGCT57290.18841025354317503No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC56100.18449668744583905No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT55860.18370739680435952No Hit
GGATTATCTCCAGGAACCTCCCACCTCTTCATTCCAGCCTCTGACAAACT54230.17834679786431107No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT54230.17834679786431107No Hit
GGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGGGTGCTGG54170.1781494752039412No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC54050.17775482988320143No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG53490.1759131517197492No Hit
GGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGC52420.17239423094315298No Hit
GAGCAAAAGCCCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGG52030.17111163365074877No Hit
GCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTCC51290.1686779875061869No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC50870.1672967288835977No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG50740.1668691964527963No Hit
GACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCT49600.16312006590576855No Hit
GGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCC48020.15792390251602836No Hit
GAGGAACCTCCCACCTCTTCATTCCAGCCTCTGACAAACTCTGCACATTA46330.15236598091561004No Hit
GCAGCAACCTGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGACTCTGCC45520.14970212500061664No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC45520.14970212500061664No Hit
GACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGG45020.1480577694975343No Hit
GGAGGCCCAAATAGCTGAAGAGGTGGAGACGTTACAGAAACCACCTGGAG44460.14621609133408206No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC44400.14601876867371216No Hit
GGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTG43700.14371667096939691No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT43640.14351934830902702No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC43420.14279583188767078No Hit
GGCTTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGT42980.1413487990449583No Hit
GGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGG42430.13954000799156774No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA42350.13927691111107457No Hit
GGAGCAGGGAGATGCTTAGTCCTGACCTGCCTGACTCTGCCTGGAACACC42120.1385205075796567No Hit
GAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCC41940.13792853959854703No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG41200.13549489345398516No Hit
GGACTCTGCTCTCTGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGT41160.1353633450137386No Hit
CCCCAAGAGTTCTGTGTGTAAGCAGCTTCTGGAAGGAAGGGCCCACACCA40260.13240350510819038No Hit
CGTCTGGGCGGAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCT40080.1318115371270807No Hit
GGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATG39430.1296738749730737No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG39220.1289832456617791No Hit
CAGGGCCAGGGTAGTGATGGGGGCTGTGGCTTCTCTATAAGGACATGCCC39130.12868726167122427No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC39020.12832550346054616No Hit
GAAGCCTGTCTTGATTATCTCCAGGAACCTCCCACCTCTTCATTCCAGCC37270.12257025919975796No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT36170.1189526770929768No Hit
GCTGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAG35820.11780162824081915No Hit
ATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCTG35670.11730832158989445No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG35560.11694656337921633No Hit
GATCCTTCCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCC35100.11543375631638059No Hit
GGAGCTGAGTCTGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCTGGG34820.11451291723465445No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT34510.11349341682274341No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT34430.11323031994225022No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC34360.11300011017181871No Hit
GGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCCCTCTGTCTCC34280.11273701329132552No Hit
GGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGGGT33150.10902076985435945No Hit
ACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGG32800.1078697210022018No Hit
GAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGAC32730.10763951123177026No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT32250.10606092994881122No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC31600.10392326779480417No Hit
GGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGA31530.10369305802437265No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC31150.10244334784203005No Hit
ACTTGGGGAGGCAGGAGTGGAAGGCAGCAGGTCGCGGTTTTCCTTCCAGT30820.10135807320999571No Hit
GAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCC30480.10023991146789972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)