FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005774750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58481
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG25554.368940339597476No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC24684.220174073630751No Hit
ATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC22923.919221627537149No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC20143.443853559275662No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA16872.8846975940903885No Hit
ATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCT13282.2708230023426412No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC7971.3628357928216002No Hit
AGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC7271.2431387972161898No Hit
GAGTGGATTTGTCATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACAT6921.1832902994134846No Hit
TCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGC6771.1576409432123254No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA6561.1217318445307023No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG5590.9558660077632052No Hit
TGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG5560.9507361365229733No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG5450.9319266086421231No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT5210.8908876387202681No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT5190.8874677245601136No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT5160.8823378533198817No Hit
TGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC5110.8737880679194951No Hit
TGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA5050.8635283254390315No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC4910.8395889263179493No Hit
CTCAGGTCTCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCT4840.8276192267574084No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA4790.819069441357022No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA4510.7711906431148577No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG3630.6207144200680563No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT3570.6104546775875925No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC3500.5984849780270516No Hit
GCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC3500.5984849780270516No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG3480.595065063866897No Hit
CGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGGCCC3410.5830953643063559No Hit
TGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG3390.5796754501462014No Hit
CCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCG3380.577965493066124No Hit
CTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCA3380.577965493066124No Hit
TGATCGATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACA3350.5728356218258922No Hit
GCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGC3140.5369265231442691No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC3110.5317966519040371No Hit
TCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC3110.5317966519040371No Hit
ACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC3020.5164070381833417No Hit
GCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGGG3010.5146970811032643No Hit
TCGATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGA2900.4958875532224141No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA2770.4736581111814094No Hit
ATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGGCTGCTCCCTCCC2720.4651083257810229No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG2550.436039055419709No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG2350.4018399138181632No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG2280.38987021425762214No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG2210.37790051469708114No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA2120.3625109009763855No Hit
AGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCTGTG2090.3573810297361536No Hit
TCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC2080.35567107265607634No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT2050.35054120141584444No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT1930.33002171645491696No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC1860.31805201689437596No Hit
CAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGG1810.30950223149398953No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC1750.2992424890135258No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC1720.2941126177732939No Hit
CCAGGCAGAGAAAGGCAGCCATCCTGCTGTCAGGGAGCTAAGACTTGCCC1700.2906927036131393No Hit
GGGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATG1700.2906927036131393No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1570.26846326157213457No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC1510.25820351909167083No Hit
AGGTCGCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCTTGG1440.24623381953112977No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA1420.24281390537097522No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC1320.22571433457020232No Hit
TTACTCAGCTTCTGAGGGTACCACTGACAAAGGAGAAGTCCCCAATGGCT1310.224004377490125No Hit
CGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAGAGC1290.22058446332997042No Hit
GAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGG1270.21716454916981587No Hit
CTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGT1260.21545459208973855No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA1200.2051948496092748No Hit
CTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCC980.16757579384757443No Hit
GCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC970.16586583676749714No Hit
GTCAAGTCAGGCCCCAAAGCTGCTGTTCCACTACTATGACAAAGATTTTA910.1556060942870334No Hit
GATTCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGC900.1538961372069561No Hit
TTTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC890.15218618012687882No Hit
CAATGCTCCAAAAACTCATCCTGTACCTTGGAGATCCAGTCCACGGAGTC860.14705630888664695No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC850.14534635180656966No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA830.14192643764641508No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC820.1402164805663378No Hit
CCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGC810.1385065234862605No Hit
ATGCTCCAAAAACTCATCCTGTACCTTGGAGATCCAGTCCACGGAGTCAG800.1367965664061832No Hit
ATTTTCTGCAGAGAGGCGCAAAGGAGTAGACTCCACTCTCAAGATCCAGC800.1367965664061832No Hit
TTATACTTCTATTGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGA760.12995673808587405No Hit
GCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC750.12824678100579676No Hit
CCTGGGAGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGC730.12482686684564218No Hit
GGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG640.10943725312494655No Hit
TAAGGATCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCA630.10772729604486928No Hit
CGGTCGCCAGTTCCCTAATTATAGCTCTGAGCTGAATGTGAACGCCTTGG620.10601733896479199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG100.007073913145.04
CTTTTCA100.007073913145.01
GTTGATA100.007073913145.08
ACAAAGT500.0145.03
TTGTCAT950.0145.09
GGTGAGA100.007073913145.0145
GGTGACA100.007073913145.0145
GAGCGAG100.007073913145.09
GGGTTGC400.0145.09
GTCATGC253.6672645E-8145.02
CAGTTCG151.2241342E-4145.08
TACAGTG100.007073913145.08
CCAGTTG100.007073913145.04
AACGATT800.0145.03
AATTCTC600.0145.06
AATTCTA306.4028427E-10145.02
TTACTCA253.6672645E-8145.01
TGCCGAG202.1136875E-6145.07
CCGCTTC100.007073913145.01
GCGGTTC202.1136875E-6145.01
Produced by FastQC (version 0.11.5)