FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005774755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005774755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922576
Sequences flagged as poor quality0
Sequence length115-124
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT2664839.118086236251855No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT802292.745146747253108No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG766722.623439048291644No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT676382.314328181713666No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT583151.9953287784475064No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG568221.9442437082902206No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT434391.486325761930571No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG343441.1751276955671983No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC339171.1605172970694346No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT318731.0905789960637464No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC265460.9083082869359086No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT262860.8994120255555373No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT262400.8978380716190102No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG247700.8475399784299879No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG231350.7915961809034222No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC223170.7636071739451771No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT194340.6649613217928293No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG182140.6232173260849333No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG178560.6109678584919606No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG174240.5961863780445744No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG173680.5942702602088021No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC171810.5878717952929197No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC171540.586947952764958No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA163430.5591984605361845No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC163100.5580693196686758No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA159040.5441774653593269No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT155620.5324754600051462No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG134600.4605526083838367No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC125210.42842341824472663No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA115320.39458340860939123No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG111740.38233394101641843No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG111310.38086263624966465No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT110410.37778316115645927No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT107950.369365929235031No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC97250.3327543920158107No Hit
GGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC96390.3298117824823033No Hit
AGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCTG95190.3257058156913627No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC90730.3104453057850335No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT89060.30473116866764116No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC88690.30346516224043446No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC88050.30127531328526613No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC87910.30079628382632306No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT87580.2996671429588144No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC82820.28338014135475004No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA82330.2817035382484493No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT80260.2746207455340768No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT78600.26894082480660897No Hit
ACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGG78580.26887239202675994No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC75800.25936023562774757No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG74680.255527999956203No Hit
GGAGCCCCACCAGGGAGACCCAGTGACATAGATGGTCTGCTCAGGGTGAT73180.2503955414675273No Hit
GGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATG73180.2503955414675273No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC73090.25008759395820673No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC71580.24492091907960648No Hit
GTCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG71550.24481826990983296No Hit
GAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGGC70850.24242312261511761No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC70690.24187566037632552No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT70180.2401306244901758No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT69950.2393436475219122No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT69880.23910413279244064No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC69770.2387277525032711No Hit
GAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATGCACAGACC68500.23438227098285896No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC68160.2332189137254258No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG65750.22497276375362008No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG65230.22319351147754582No Hit
GCCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCC62930.21532374179490968No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC62720.2146051976064951No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT58050.19862614351175129No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC57280.1959914814875644No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC56710.1940411472618676No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC56540.19345946863315103No Hit
GAGAGTATAGCTGGTTCCCAGGGAGGCTGGGGGTGATTCACCACACTCTT56500.193322603073453No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG56100.1919539474764728No Hit
GGAATGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACC56080.1918855146966238No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG55680.1905168590996436No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT52870.18090205353085773No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC52250.1787806373555384No Hit
GACACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC52160.17847268984621786No Hit
GAATGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCA51700.17689873590969063No Hit
GCTATTTCCCCAGGCAGGGCTGGGAGAGATGAGATCCTGGCCTGGACCTG51390.17583802782203098No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC50540.17292963467844807No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT49280.16861836954796042No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC49140.16813934008901738No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC48060.1644439699771708No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG47710.16324639632981316No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT47240.16163822600336142No Hit
ACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATG47070.16105654737464484No Hit
GGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGC45540.15582143971619558No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT45000.15397375466027233No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG44160.15109957790661388No Hit
GAGAATGAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCAC43330.14825961754287997No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG43180.14774637169401242No Hit
GAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGGGTCCTG42320.144803762160505No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGCCCCTGCCAT41950.1435377557332983No Hit
GGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCC40940.1400819003509233No Hit
GGACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCA40730.1393633561625087No Hit
GGAAAGACGTGTTCCCTTTTCACCAATGCACAGACCCAGAGGACCCCTCC40590.13888432670356562No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT40230.13765253666628344No Hit
GGCAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGGGTCC40010.13689977608794432No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC39950.1366944777483973No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT39570.1353942549312661No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG38430.13149358647987253No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC37840.12947481947432676No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC37730.1290984391851572No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG37520.1283798949967426No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG37380.12790086553779953No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC37230.12738761968893195No Hit
GATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCTATGTGGCC37130.1270454557896869No Hit
GAGCTGTAGCATCAGCTTTGTCCTTCTCTGCAGGTCCAGTGAATGCTGGT36750.12574523297255571No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA36260.12406862986625497No Hit
GATTCCAGCCTCTGACAAACTCTGCACATTAGGCCAGGAGAAGCCCCCGA35950.12300792177859532No Hit
GGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATGCAC35380.12105758755289854No Hit
GGCTTCTGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTG34550.11821762718916462No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG34540.11818341079924011No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC34210.11705426993173147No Hit
GAGTCCTCTCACCACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGT33580.11489863736648764No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT31970.10938979858864234No Hit
GCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC31900.1091502838591708No Hit
GACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCT31860.10901341829947278No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT31830.10891076912969927No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCC31690.1084317396707562No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA31210.10678935295437997No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT30880.1056602120868713No Hit
GGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC30710.10507853345815472No Hit
GAGGTCTCAGAATGACGCCCTTGAAAGACGTGTTCCCTTTTCACCAATGC30690.10501010067830571No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT30670.1049416678984567No Hit
GGATTCACCACACTCTTAAAAGAAGACTAGCCTCATTTCCTCCCCAGCTC30620.10477058594883418No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT30380.10394939259064605No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC30290.10364144508132553No Hit
GATCCTGGCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGTGGTC30110.10302555006268443No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC30090.10295711728283542No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG29700.10162267807577972No Hit
GCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGC29600.10128051417653468No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC29580.10121208139668567No Hit
CAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG29380.10052775359819556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)